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学科主题: 高分子化学
题名: Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization
其他题名: Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization
作者: Sheng RL(盛瑞隆)1; AN FEIFEI1; Wang Z(王昭)1; LI MINGRUI1; Cao AM(曹阿民)1
通讯作者: 盛瑞隆 ; 曹阿民
刊名: RSC Adv.
发表日期: 2015
DOI: 10.1039/c4ra06879c
卷: 5, 期:16, 页:12338-12345
收录类别: SCI
文章类型: 论文
英文摘要: In this study, we constructed a visible model for drug/gene dual delivery. Firstly, we prepared a series of pyrene fluorophore bearing cationic amphiphiles (Py-amines), which could be further employed as amphiphilic gene carriers and antitumor polyamine drug models. Then, the Py-amines fluorescent amphiphiles were utilized to bind/load plasmid DNA via electrostatic interactions to form nano-sized particles in aqueous solution. The average size, zeta potential and morphology of the self-assembled Pyamines/ pDNA complexes were found to be largely dependent on the molecular structures of Py-amines amphiphiles. Moreover, the Py-amines and their pDNA complexes showed an evident cell proliferation inhibition capability in H1299 (human lung cancer) cells. Notably, the lysosomal localization of the Pyamines/ pDNA complexes could be directly visualized using fluorescence microscopy in vitro. In summary, this current study could provide new and visible approach to design polyamine-based antitumor drug/plasmid DNA dual delivery systems, which could facilitate a greater understanding of the intracellular trafficking/localization of polyamine-based cationic gene/drug payloads.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000348985400081
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39837
Appears in Collections:高分子材料研究室_期刊论文

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作者单位: 1.中科院上海有机化学研究所, 有机功能分子合成与组装化学重点实验室
2.国家纳米科学中心

Recommended Citation:
Sheng RL,AN FEIFEI,Wang Z,et al. Assembly of plasmid DNA with pyrene-amines cationic amphiphiles into nanoparticles and their visible lysosome localization[J]. RSC Adv.,2015,5(16):12338-12345.
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