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学科主题: 分析化学
题名: Behaviors of Leucine and Isoleucine in Ion Mobility-Quadrupole Time of Flight Mass Spectrometry
其他题名: 离子迁移四极杆飞行时间质谱对亮氨酸和异亮氨酸离子化行为的研究
作者: Guo S(郭甦)1; Zhang F(张芳)1; Wang HY(王昊阳)1; Zhang MY(张曼玉)1; Zhang ZX(张之旭)1; Zhang X(张祥)1; Guo YL(郭寅龙)1
通讯作者: 张芳 ; 郭寅龙
刊名: Chin. J. Chem.
发表日期: 2015
DOI: 10.1002/cjoc.201500670
卷: 33, 期:12, 页:1359-1364
收录类别: SCI
文章类型: 论文
英文摘要: In this study, ion mobility separation coupled with tandem mass spectrometry (IM-tandem MS) was utilized to investigate the ionization behaviors of two amino acids including leucine and isoleucine. Under the electrospray ionization (ESI) mode, two protonation sites in each molecular sturcture caused two forms of protomer. One arose from the amino being protonated (amino-protomer) and the other from the carboxyl being protonated (carboxyl- protomer). In the two-dimensional (drift time, m/z) spectrum, the protomers had the same mass, but the distinguishable drift times and fragmentation patterns. For the characterization purpose, the theoretical collision cross section (CCS) values of the protomers were calculated and proven to be consistent with the experimental. Moreover, the quantified relationship between the amino acids and their protomers was evaluated. It showed that the abundance of the carboxyl-protomer was proportional to the concentration of the amino acid, whereas that of the amino-protomer did not have the same trend. Under the atmospheric pressure chemical ionization (APCI) mode, only the carboxyl-protomer was observed. In addition, the amino-protomer and the cluster ions observed under ESI were absent completely. The results demonstrate that the ionization mode impacts heavily on the ionization behaviors of leucine and isoleucine not only on the form of therir protomers but also on the quantified relationship.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000368270000005
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39697
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 1.中科院上海有机化学研究所, 上海质谱中心
2.安捷伦科技(中国)有限公司
3.复旦大学
4.浙江工商大学

Recommended Citation:
Guo S,Zhang F,Wang HY,et al. Behaviors of Leucine and Isoleucine in Ion Mobility-Quadrupole Time of Flight Mass Spectrometry[J]. Chin. J. Chem.,2015,33(12):1359-1364.
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