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Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling
Alternative Title基于稳定同位素标记技术的电喷雾离子淌度质谱法的脂肪酸比较分析的开发
Leng JP(冷嘉鹏)1; Guan Q(官青)1; Sun TQ(孙团起)1; Wang HY(王昊阳)1; CUI JIANLAN1; LIU QINGHAO1; ZHANG ZHIXU1; Zhang ZX(张之旭)1; Zhang MY(张曼玉)1; Guo YL(郭寅龙)1
2015
Source PublicationAnal. Chim. Acta
Volume887Pages:148-154
AbstractA rapid method for fatty acids (FAs) comparative profiling based on carboxyl-specific stable isotope labeling (SIL) and direct infusion electrospray ionization-ion mobility-mass spectrometry (ESI-IM-MS) is established. The design of the method takes advantage of the three-dimensional characteristics of IM -MS including drift time, m/z and ion intensity, for comparison of d0-/d6-2,4-dimethoxy-6-piperazin-1-yl pyrimidine (DMPP)-labeled FAs. In particular, without chromatographic separation, the method allowed direct FAs profiling in complex samples due to the advantageous priority of DMPP in signal enhancement as well as the extra resolution that IM-MS offered. Additionally, the d0-/d6-DMPP-labeled FAs showed expected features, including very similar drift times, 6 Da mass deviations, specific reporter ions, similar MS responses, and adherence to the drift time rule regarding the influence of carbon chain length and unsaturation on relative drift times. Therefore, the introduction of isotope analogs minimized the matrix effect and variations in quantification and ensured accurate identification of non-targeted FAs by those typical features. Peak intensity ratios between d0-/d6-DMPP-labeled ions were subsequently used in relative quantification for the detected FAs. The established strategy has been applied successfully in the rapid profiling of trace free FAs between normal and cancerous human thyroid tissues. Sixteen free FAs were found with the increased level with a statistically significant difference (p < 0.05) compared to the normal tissue samples. The integrated SIL technique and ESI-IM-MS are expected to serve as an alternative tool for high-throughput analysis of FAs in complex samples. (C) 2015 Elsevier B.V. All rights reserved.
Subtype论文
Subject Area分析化学
DOI10.1016/j.aca.2015.06.029
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000360644100016
Citation statistics
Cited Times:15[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39693
Collection分析化学研究室
Corresponding AuthorLeng JP(冷嘉鹏); Sun TQ(孙团起); Guo YL(郭寅龙)
Affiliation1.中北大学
2.复旦大学上海癌症中心
3.复旦大学上海医科大学
4.中科院上海有机化学研究所, 上海质谱中心
5.安捷伦科技(中国)有限公司
Recommended Citation
GB/T 7714
Leng JP,Guan Q,Sun TQ,et al. Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling[J]. Anal. Chim. Acta,2015,887:148-154.
APA 冷嘉鹏.,官青.,孙团起.,王昊阳.,CUI JIANLAN.,...&郭寅龙.(2015).Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling.Anal. Chim. Acta,887,148-154.
MLA 冷嘉鹏,et al."Direct infusion electrospray ionization-ion mobility-mass spectrometry for comparative profiling of fatty acids based on stable isotope labeling".Anal. Chim. Acta 887(2015):148-154.
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