中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 分析化学研究室  > 期刊论文
学科主题: 分析化学
题名: Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry
其他题名: 毛细管电泳结合高效液相色谱-质谱联用用于天然产物中表皮生长因子受体抑制剂的筛选
作者: Li F(李凤)1; Zhang YM(张艳梅)1; QIU DANYE1; Kang JW(康经武)1
通讯作者: 康经武
刊名: J. Chromatogr. A
发表日期: 2015
DOI: 10.1016/j.chroma.2015.04.055
卷: 1400, 页:117-123
收录类别: SCI
文章类型: 论文
英文摘要: A method for screening of inhibitors to epidermal growth factor receptor (EGFR) in natural product extracts with capillary electrophoresis (CE) in conjunction with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is reported. The method was established by employing 5-carboxyfluorescein labeled substrate peptide, two commercially available EGFR inhibitors OSI-744 and ZD1839, and a small chemical library consisted of 39 natural product extracts derived from the Traditional Chinese Medicines. Biochemical assay of crude natural product extracts was carried out by using CE equipped with a laser induced fluorescence detector. The CE separation allowed an accurately quantitative measurement of the phosphorylated product, hence the measurement of the enzymatic activity as well as the inhibition kinetics. The hits are identified if the peak area of the phosphorylated product is reduced in comparison with the negative control. The active constituents in the natural product extract were then identified by an assay-guided isolation with HPLC-MS/MS system. With the method, the flavonoids component of the Lycopus lucidus extract, namely quercetin and rutin were identified to be the active ingredients. Their IC50 values were determined as 0.88 mu M and 10.1 mu M, respectively. This result demonstrated a significant merit of our method in the identification of the bioactive compounds in natural products. (C) 2015 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000356742500014
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39691
Appears in Collections:分析化学研究室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2015337.pdf(620KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.中科院上海有机化学研究所, 生命有机化学国家重点实验室
2.上海科技大学

Recommended Citation:
Li F,Zhang YM,QIU DANYE,et al. Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2015,1400:117-123.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[李凤]'s Articles
[张艳梅]'s Articles
[QIU DANYE]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[李凤]‘s Articles
[张艳梅]‘s Articles
[QIU DANYE]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2015337.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace