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Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry
其他题名毛细管电泳结合高效液相色谱-质谱联用用于天然产物中表皮生长因子受体抑制剂的筛选
Li F(李凤)1; Zhang YM(张艳梅)1; QIU DANYE1; Kang JW(康经武)1
2015
发表期刊J. Chromatogr. A
卷号1400页码:117-123
摘要A method for screening of inhibitors to epidermal growth factor receptor (EGFR) in natural product extracts with capillary electrophoresis (CE) in conjunction with high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) is reported. The method was established by employing 5-carboxyfluorescein labeled substrate peptide, two commercially available EGFR inhibitors OSI-744 and ZD1839, and a small chemical library consisted of 39 natural product extracts derived from the Traditional Chinese Medicines. Biochemical assay of crude natural product extracts was carried out by using CE equipped with a laser induced fluorescence detector. The CE separation allowed an accurately quantitative measurement of the phosphorylated product, hence the measurement of the enzymatic activity as well as the inhibition kinetics. The hits are identified if the peak area of the phosphorylated product is reduced in comparison with the negative control. The active constituents in the natural product extract were then identified by an assay-guided isolation with HPLC-MS/MS system. With the method, the flavonoids component of the Lycopus lucidus extract, namely quercetin and rutin were identified to be the active ingredients. Their IC50 values were determined as 0.88 mu M and 10.1 mu M, respectively. This result demonstrated a significant merit of our method in the identification of the bioactive compounds in natural products. (C) 2015 Elsevier B.V. All rights reserved.
文章类型论文
学科领域分析化学
DOI10.1016/j.chroma.2015.04.055
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000356742500014
引用统计
被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/39691
专题分析化学研究室
通讯作者Kang JW(康经武)
作者单位1.中科院上海有机化学研究所, 生命有机化学国家重点实验室
2.上海科技大学
推荐引用方式
GB/T 7714
Li F,Zhang YM,QIU DANYE,et al. Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2015,1400:117-123.
APA 李凤,张艳梅,QIU DANYE,&康经武.(2015).Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry.J. Chromatogr. A,1400,117-123.
MLA 李凤,et al."Screening of epidermal growth factor receptor inhibitors in natural products by capillary electrophoresis combined with high performance liquid chromatography-tandem mass spectrometry".J. Chromatogr. A 1400(2015):117-123.
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