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Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection
Alternative Title增强的毛细管电泳间接紫外检测用于肝素中游离硫酸根离子的测定
Zhang YM(张艳梅); QIU DANYE; Kang JW(康经武)
2015
Source PublicationChromatographia
Volume78Issue:11-12Pages:833-837
AbstractA simple, sensitive and environmentally friendly capillary electrophoresis method for the determination of free sulfate anions in heparin and low-molecular-weight heparin with indirect UV detection is reported. The background electrolyte consists of 20 mM nitrate (pH 5.5) containing 0.2 mM CTAB. The use of nitrate as the probe anion is based on the consideration that nitrate has a very close electrophoretic mobility to that of sulfate, leading to a very narrow peak shape. Moreover, the apparent molar absorptivity of nitrate at 202 nm (8413 L mol(-1) cm(-1)) is much higher than that of the commonly used probe anion chromate at 254 nm (2400 L mol(-1) cm(-1)). The combination of the narrow peak shape with the high apparent molar absorptivity of the probe ion improved the limit of detection by 2.4 times and the limit of quantitation by 3.2 times. The effect of various CE parameters on the separation of sulfate from chloride was investigated and optimized. The method displays linearity in the range from 0.05 to 20 mM. The RSD % for intraday (n = 6), and interday (n = 3) repeatability in terms of migration times and peak areas were all less than 3 %. The limit of detection (S/N = 3) and limit of quantitation (S/N = 10) for sulfate were determined as 18 and 53 A mu M, respectively. The method was successfully applied for the quantification of sulfate anions in heparin and low-molecular-weight heparins.
Subtype论文
Subject Area分析化学
DOI10.1007/s10337-015-2906-y
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000354904100010
Citation statistics
Cited Times:4[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39690
Collection分析化学研究室
Corresponding AuthorKang JW(康经武)
Affiliation中科院上海有机化学研究所
Recommended Citation
GB/T 7714
Zhang YM,QIU DANYE,Kang JW. Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection[J]. Chromatographia,2015,78(11-12):833-837.
APA 张艳梅,QIU DANYE,&康经武.(2015).Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection.Chromatographia,78(11-12),833-837.
MLA 张艳梅,et al."Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection".Chromatographia 78.11-12(2015):833-837.
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