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Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection
其他题名增强的毛细管电泳间接紫外检测用于肝素中游离硫酸根离子的测定
Zhang YM(张艳梅); QIU DANYE; Kang JW(康经武)
2015
发表期刊Chromatographia
卷号78期号:11-12页码:833-837
摘要A simple, sensitive and environmentally friendly capillary electrophoresis method for the determination of free sulfate anions in heparin and low-molecular-weight heparin with indirect UV detection is reported. The background electrolyte consists of 20 mM nitrate (pH 5.5) containing 0.2 mM CTAB. The use of nitrate as the probe anion is based on the consideration that nitrate has a very close electrophoretic mobility to that of sulfate, leading to a very narrow peak shape. Moreover, the apparent molar absorptivity of nitrate at 202 nm (8413 L mol(-1) cm(-1)) is much higher than that of the commonly used probe anion chromate at 254 nm (2400 L mol(-1) cm(-1)). The combination of the narrow peak shape with the high apparent molar absorptivity of the probe ion improved the limit of detection by 2.4 times and the limit of quantitation by 3.2 times. The effect of various CE parameters on the separation of sulfate from chloride was investigated and optimized. The method displays linearity in the range from 0.05 to 20 mM. The RSD % for intraday (n = 6), and interday (n = 3) repeatability in terms of migration times and peak areas were all less than 3 %. The limit of detection (S/N = 3) and limit of quantitation (S/N = 10) for sulfate were determined as 18 and 53 A mu M, respectively. The method was successfully applied for the quantification of sulfate anions in heparin and low-molecular-weight heparins.
文章类型论文
学科领域分析化学
DOI10.1007/s10337-015-2906-y
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收录类别SCI
语种英语
WOS记录号WOS:000354904100010
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被引频次:4[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/39690
专题分析化学研究室
通讯作者Kang JW(康经武)
作者单位中科院上海有机化学研究所
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GB/T 7714
Zhang YM,QIU DANYE,Kang JW. Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection[J]. Chromatographia,2015,78(11-12):833-837.
APA 张艳梅,QIU DANYE,&康经武.(2015).Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection.Chromatographia,78(11-12),833-837.
MLA 张艳梅,et al."Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection".Chromatographia 78.11-12(2015):833-837.
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