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学科主题: 分析化学
题名: Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection
其他题名: 增强的毛细管电泳间接紫外检测用于肝素中游离硫酸根离子的测定
作者: Zhang YM(张艳梅); QIU DANYE; Kang JW(康经武)
通讯作者: 康经武
刊名: Chromatographia
发表日期: 2015
DOI: 10.1007/s10337-015-2906-y
卷: 78, 期:11-12, 页:833-837
收录类别: SCI
文章类型: 论文
英文摘要: A simple, sensitive and environmentally friendly capillary electrophoresis method for the determination of free sulfate anions in heparin and low-molecular-weight heparin with indirect UV detection is reported. The background electrolyte consists of 20 mM nitrate (pH 5.5) containing 0.2 mM CTAB. The use of nitrate as the probe anion is based on the consideration that nitrate has a very close electrophoretic mobility to that of sulfate, leading to a very narrow peak shape. Moreover, the apparent molar absorptivity of nitrate at 202 nm (8413 L mol(-1) cm(-1)) is much higher than that of the commonly used probe anion chromate at 254 nm (2400 L mol(-1) cm(-1)). The combination of the narrow peak shape with the high apparent molar absorptivity of the probe ion improved the limit of detection by 2.4 times and the limit of quantitation by 3.2 times. The effect of various CE parameters on the separation of sulfate from chloride was investigated and optimized. The method displays linearity in the range from 0.05 to 20 mM. The RSD % for intraday (n = 6), and interday (n = 3) repeatability in terms of migration times and peak areas were all less than 3 %. The limit of detection (S/N = 3) and limit of quantitation (S/N = 10) for sulfate were determined as 18 and 53 A mu M, respectively. The method was successfully applied for the quantification of sulfate anions in heparin and low-molecular-weight heparins.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000354904100010
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39690
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 中科院上海有机化学研究所

Recommended Citation:
Zhang YM,QIU DANYE,Kang JW. Determination of Sulfate Anions in Heparin by Capillary Electrophoresis with Improved Indirect UV Detection[J]. Chromatographia,2015,78(11-12):833-837.
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