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学科主题: 分析化学
题名: Screening of mammalian target of rapamycin inhibitors in natural product extracts by capillary electrophoresis in combination with high performance liquid chromatography-tandem mass spectrometry
其他题名: 毛细管电泳结合高效液相色谱-质谱联用用于天然产物中雷帕霉素靶标蛋白抑制剂的筛选
作者: Zhang YM(张艳梅)1; Li F(李凤)1; LI MINGXIA1; Kang JW(康经武)1
通讯作者: 康经武
刊名: J. Chromatogr. A
发表日期: 2015
DOI: 10.1016/j.chroma.2015.02.033
卷: 1388, 页:267-273
收录类别: SCI
文章类型: 论文
英文摘要: In this study, capillary electrophoresis (CE) combined with HPLC-MS/MS were used as a powerful platform for screening of inhibitors of mammalian target of rapamycin (mTOR) in natural product extracts. The screening system has been established by using 5-carboxyfluorescein labeled substrate peptide F-4EBP1, a known mTOR inhibitor AZD8055, and a small chemical library consisted of 18 natural product extracts. Biochemical screening of natural product extracts was performed by CE with laser induced fluorescence detection. The CE separation allowed a quantitative measurement of the phosphorylated product, hence the quantitation of enzymatic inhibition as well as inhibition kinetics. The hits are readily identified as long as the peak area of the phosphorylated product is reduced in comparison with the negative control. Subsequent assay-guided isolation of the active natural product extract was performed with HPLC-MS/MS to track the particular active components. The structures of the identified active components were elucidated by the molecular ions and fragmentaion information provided by MS/MS analysis. The CE-based assay method only requires minute pure compounds, which can be readily purified by HPLC. Therefore, the combination of CE and HPLC-MS/MS provides a high-throughput platform for screening bioactive compounds from the crude nature extracts. By taking the advantage of the screening system, salvianolic acid A and C in extract of Salvia miltiorrhiza were discovered as the new mTOR inhibitors. (C) 2015 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000351792400031
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39689
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 1.中科院上海有机化学研究所, 生命有机化学国家重点实验室
2.上海科技大学

Recommended Citation:
Zhang YM,Li F,LI MINGXIA,et al. Screening of mammalian target of rapamycin inhibitors in natural product extracts by capillary electrophoresis in combination with high performance liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2015,1388:267-273.
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