SIOC OpenIR  > 分析化学研究室
Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry
Alternative Title邻苯二甲酸作为添加剂在提高甲状腺组织中氨基酸的液质检测灵敏度中的应用
Qi WS(祁婉舒)1; Guan Q(官青)1; Sun TQ(孙团起)1; CAO YANJING1; Zhang L(张立)1; Guo YL(郭寅龙)1
2015
Source PublicationAnal. Chim. Acta
Volume870Pages:75-82
AbstractIn this work, 0.08 mmol L-1 of phthalic acid was introduced as a mobile phase additive to quantify free amino acids (AAs) by hydrophilic interaction liquid chromatography (HILIC) coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS). The addition of phthalic acid significantly increased the signal intensity of protonated AA ions, resulting from the decrease of the relative abundance of AA sodium adducts. Meanwhile, the chromatographic peak shapes of AAs were optimized. As a consequence, there was a noticeable increase in the sensitivity of detection for AAs. The limits of detection (LOD) and quantification (LOQ) of the AAs ranged from 0.0500 to 20.0 ng mL (-1) and from 0.100 to 50.0 ng mL(-1), respectively, which were 4-50 times lower compared to the values measured without the addition of phthalic acid. The enhanced detection and separation of AAs were obtained by merely adding phthalic acid to the mobile phase without changing other conditions. Eventually, this simple method was validated and successfully applied to the analysis of twenty-four kinds of free AAs in human thyroid carcinoma and para-carcinoma tissues, demonstrating a significant increase of most AAs in thyroid carcinoma tissues (p < 0.05). (C) 2015 Elsevier B.V. All rights reserved.
Subtype论文
Subject Area分析化学
DOI10.1016/j.aca.2015.02.048
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000351778600007
Citation statistics
Cited Times:24[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39688
Collection分析化学研究室
Corresponding AuthorSun TQ(孙团起); Zhang L(张立); Guo YL(郭寅龙)
Affiliation1.中科院上海有机化学研究所, 上海质谱中心
2.复旦大学, 上海癌症中心
3.复旦大学, 上海医科大学
Recommended Citation
GB/T 7714
Qi WS,Guan Q,Sun TQ,et al. Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry[J]. Anal. Chim. Acta,2015,870:75-82.
APA 祁婉舒,官青,孙团起,CAO YANJING,张立,&郭寅龙.(2015).Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry.Anal. Chim. Acta,870,75-82.
MLA 祁婉舒,et al."Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry".Anal. Chim. Acta 870(2015):75-82.
Files in This Item:
File Name/Size DocType Version Access License
2015161.pdf(1129KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[祁婉舒]'s Articles
[官青]'s Articles
[孙团起]'s Articles
Baidu academic
Similar articles in Baidu academic
[祁婉舒]'s Articles
[官青]'s Articles
[孙团起]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[祁婉舒]'s Articles
[官青]'s Articles
[孙团起]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.