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学科主题: 分析化学
题名: Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry
其他题名: 邻苯二甲酸作为添加剂在提高甲状腺组织中氨基酸的液质检测灵敏度中的应用
作者: Qi WS(祁婉舒)1; Guan Q(官青)1; Sun TQ(孙团起)1; CAO YANJING1; Zhang L(张立)1; Guo YL(郭寅龙)1
通讯作者: 孙团起 ; 张立 ; 郭寅龙
刊名: Anal. Chim. Acta
发表日期: 2015
DOI: 10.1016/j.aca.2015.02.048
卷: 870, 页:75-82
收录类别: SCI
文章类型: 论文
英文摘要: In this work, 0.08 mmol L-1 of phthalic acid was introduced as a mobile phase additive to quantify free amino acids (AAs) by hydrophilic interaction liquid chromatography (HILIC) coupled with electrospray ionization tandem mass spectrometry (ESI-MS/MS). The addition of phthalic acid significantly increased the signal intensity of protonated AA ions, resulting from the decrease of the relative abundance of AA sodium adducts. Meanwhile, the chromatographic peak shapes of AAs were optimized. As a consequence, there was a noticeable increase in the sensitivity of detection for AAs. The limits of detection (LOD) and quantification (LOQ) of the AAs ranged from 0.0500 to 20.0 ng mL (-1) and from 0.100 to 50.0 ng mL(-1), respectively, which were 4-50 times lower compared to the values measured without the addition of phthalic acid. The enhanced detection and separation of AAs were obtained by merely adding phthalic acid to the mobile phase without changing other conditions. Eventually, this simple method was validated and successfully applied to the analysis of twenty-four kinds of free AAs in human thyroid carcinoma and para-carcinoma tissues, demonstrating a significant increase of most AAs in thyroid carcinoma tissues (p < 0.05). (C) 2015 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000351778600007
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39688
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 1.中科院上海有机化学研究所, 上海质谱中心
2.复旦大学, 上海癌症中心
3.复旦大学, 上海医科大学

Recommended Citation:
Qi WS,Guan Q,Sun TQ,et al. Improving detection sensitivity of amino acids in thyroid tissues by using phthalic acid as a mobile phase additive in hydrophilic interaction chromatography-electrospray ionization-tandem mass spectrometry[J]. Anal. Chim. Acta,2015,870:75-82.
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