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Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis
其他题名以结构为基础工程改造抗霉素生物合成中的巴豆酰CoA羧化酶/还原酶以合理控制聚酮的延伸单元
Zhang LH(张骊駻)1; MORI TAKAHIRO1; Zheng QF(郑庆飞)1; AWAKAWA TAKAYOSHI1; Yan Y(闫岩)1; Liu W(刘文)1; ABE IKURO1
2015
发表期刊Angew. Chem.-Int. Edit.
卷号54期号:45页码:13462-13465
摘要Bioengineering of natural product biosynthesis is a powerful approach to expand the structural diversity of bioactive molecules. However, in polyketide biosynthesis, the modification of polyketide extender units, which form the carbon skeletons, has remained challenging. Herein, we report the rational control of polyketide extender units by the structure-based engineering of a crotonyl-CoA carboxylase/reductase (CCR), in the biosynthesis of antimycin. Site-directed mutagenesis of the CCR enzyme AntE, guided by the crystal structure solved at 1.5 angstrom resolution, expanded its substrate scope to afford indolylmethylmalonyl-CoA by the V350G mutation. The mutant A182L selectively catalyzed carboxylation over the regular reduction. Furthermore, the combinatorial biosynthesis of heterocycle- and substituted arene-bearing antimycins was achieved by an engineered Streptomyces strain bearing AntE(V350G). These findings deepen our understanding of the molecular mechanisms of the CCRs, which will serve as versatile biocatalysts for the manipulation of building blocks, and set the stage for the rational design of polyketide biosynthesis.
文章类型论文
学科领域生命有机化学
DOI10.1002/anie.201506899
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000363691500059
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被引频次:9[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/39532
专题生命有机化学国家重点实验室
通讯作者ABE IKURO
作者单位1.日本东京大学
2.中科院上海有机化学研究所, 生命有机化学国家重点实验室
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Zhang LH,MORI TAKAHIRO,Zheng QF,et al. Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis[J]. Angew. Chem.-Int. Edit.,2015,54(45):13462-13465.
APA 张骊駻.,MORI TAKAHIRO.,郑庆飞.,AWAKAWA TAKAYOSHI.,闫岩.,...&ABE IKURO.(2015).Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis.Angew. Chem.-Int. Edit.,54(45),13462-13465.
MLA 张骊駻,et al."Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis".Angew. Chem.-Int. Edit. 54.45(2015):13462-13465.
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