中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 生命有机化学国家重点实验室  > 期刊论文
学科主题: 生命有机化学
题名: Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis
其他题名: 以结构为基础工程改造抗霉素生物合成中的巴豆酰CoA羧化酶/还原酶以合理控制聚酮的延伸单元
作者: Zhang LH(张骊駻)1; MORI TAKAHIRO1; Zheng QF(郑庆飞)1; AWAKAWA TAKAYOSHI1; Yan Y(闫岩)1; Liu W(刘文)1; ABE IKURO1
通讯作者: ABE IKURO
刊名: Angew. Chem.-Int. Edit.
发表日期: 2015
DOI: 10.1002/anie.201506899
卷: 54, 期:45, 页:13462-13465
收录类别: SCI
文章类型: 论文
英文摘要: Bioengineering of natural product biosynthesis is a powerful approach to expand the structural diversity of bioactive molecules. However, in polyketide biosynthesis, the modification of polyketide extender units, which form the carbon skeletons, has remained challenging. Herein, we report the rational control of polyketide extender units by the structure-based engineering of a crotonyl-CoA carboxylase/reductase (CCR), in the biosynthesis of antimycin. Site-directed mutagenesis of the CCR enzyme AntE, guided by the crystal structure solved at 1.5 angstrom resolution, expanded its substrate scope to afford indolylmethylmalonyl-CoA by the V350G mutation. The mutant A182L selectively catalyzed carboxylation over the regular reduction. Furthermore, the combinatorial biosynthesis of heterocycle- and substituted arene-bearing antimycins was achieved by an engineered Streptomyces strain bearing AntE(V350G). These findings deepen our understanding of the molecular mechanisms of the CCRs, which will serve as versatile biocatalysts for the manipulation of building blocks, and set the stage for the rational design of polyketide biosynthesis.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000363691500059
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39532
Appears in Collections:生命有机化学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2015544.pdf(1674KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.日本东京大学
2.中科院上海有机化学研究所, 生命有机化学国家重点实验室

Recommended Citation:
Zhang LH,MORI TAKAHIRO,Zheng QF,et al. Rational Control of Polyketide Extender Units by Structure-Based Engineering of a Crotonyl-CoA Carboxylase/Reductase in Antimycin Biosynthesis[J]. Angew. Chem.-Int. Edit.,2015,54(45):13462-13465.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[张骊駻]'s Articles
[MORI TAKAHIRO]'s Articles
[郑庆飞]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[张骊駻]‘s Articles
[MORI TAKAHIRO]‘s Articles
[郑庆飞]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2015544.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace