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Crystallographic analysis of NosA, which catalyzes terminal amide formation in the biosynthesis of nosiheptide
Alternative Title硫肽nosiheptide生物合成最后一般碳酰胺化被NosA催化形成的晶体学研究
Wang YT(王彦婷)1; Liu SS(刘珊珊)1; Me PF(么鹏飞)1; Yu Y(虞沂)1; Zhang Y(张岩)1; Lan WX(蓝文贤)1; Wang CX(王春喜)1; Ding JP(丁建平)1; Liu W(刘文)1; Cao CY(曹春阳)1
2015
Source PublicationActa Crystallogr. F-Struct. Biol. Commun.
Volume71Pages:1033-1037
AbstractNosiheptide is a member of the thiopeptide family of antibiotics which demonstrates potent activities against various bacterial pathogens. The formation of its C-terminal amide is catalysed by NosA in an unusual strategy for maturating certain thiopeptides by processing precursor peptides featuring a serine extension. Here, a recombinant C-terminally truncated selenomethionine-derivatized NosA(1-111) variant from Streptomyces actuosus consisting of residues 1-111, named SeMet NosA(1-111), was crystallized using the sitting-drop vapour-diffusion method. Diffraction data were collected to 2.40 angstrom resolution using synchrotron radiation. The crystals belonged to the primitive cubic space group P4(1)32, with unit-cell parameters a = b = c = 143.3 angstrom. Assuming the presence of three molecules in the asymmetric unit, the calculated Matthews coefficient was 3.94 angstrom(3)Da(-1) and the corresponding solvent content was 40.3%.
Subtype论文
Subject Area生命有机化学
DOI10.1107/S2053230X15011085
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000359352700019
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39519
Collection生命有机化学国家重点实验室
Corresponding AuthorCao CY(曹春阳)
Affiliation1.华中科技大学
2.中科院上海有机化学研究所, 生命有机化学国家重点实验室
3.中科院上海有机化学研究所, 生物与化学交叉研究中心
Recommended Citation
GB/T 7714
Wang YT,Liu SS,Me PF,et al. Crystallographic analysis of NosA, which catalyzes terminal amide formation in the biosynthesis of nosiheptide[J]. Acta Crystallogr. F-Struct. Biol. Commun.,2015,71:1033-1037.
APA 王彦婷.,刘珊珊.,么鹏飞.,虞沂.,张岩.,...&曹春阳.(2015).Crystallographic analysis of NosA, which catalyzes terminal amide formation in the biosynthesis of nosiheptide.Acta Crystallogr. F-Struct. Biol. Commun.,71,1033-1037.
MLA 王彦婷,et al."Crystallographic analysis of NosA, which catalyzes terminal amide formation in the biosynthesis of nosiheptide".Acta Crystallogr. F-Struct. Biol. Commun. 71(2015):1033-1037.
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