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The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis
Alternative TitleSARP家族调控蛋白Txn9和双组份调控蛋白Txn11是三欣卡辛生物合成中的正调控因子
Yang K(杨魁)1; Qi LH(漆丽华)1; Zhang M(张媚)1; Hou XF(侯现锋)1; Pan HX(潘海学)1; Tang GL(唐功利)1; Wang W(王伟)1; Yuan H(袁华)1
2015
Source PublicationCurr. Microbiol.
Volume71Issue:4Pages:458-464
AbstractTrioxacarcin A is a polyoxygenated, structurally complex antibiotic produced by Streptomyces spp., which possesses high anti-bacterial, anti-malaria, and anti-tumor activities. The trioxacarcin biosynthetic pathway involves type II polyketide synthases (PKSs) with l-isoleucine as a unique starter unit, as well as many complex post-PKS tailoring enzymes and resistance and regulatory proteins. In this work, two regulatory genes, txn9 coding for a Streptomyces antibiotic regulatory protein family regulator and txn11 for a two-component response regulator, were revealed to be absolutely required for trioxacarcin production by individually inactivating all the six annotated regulatory genes in the txn cluster. Complementation assay suggested that these two activators do not have a regulatory cascade relationship. Moreover, transcriptional analysis showed that they activate 15 of the 28 txn operons, indicating that a complicated regulatory network is involved in the trioxacarcin production. Information gained from this study may be useful for improving the production of the highly potent trioxacarcin A.
Subtype论文
Subject Area生命有机化学
DOI10.1007/s00284-015-0868-9
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000359807900005
Citation statistics
Cited Times:3[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39518
Collection生命有机化学国家重点实验室
Corresponding AuthorWang W(王伟); Yuan H(袁华)
Affiliation1.上海应用技术学院
2.中科院上海有机化学研究所, 生命有机化学国家重点实验室
Recommended Citation
GB/T 7714
Yang K,Qi LH,Zhang M,et al. The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis[J]. Curr. Microbiol.,2015,71(4):458-464.
APA 杨魁.,漆丽华.,张媚.,侯现锋.,潘海学.,...&袁华.(2015).The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis.Curr. Microbiol.,71(4),458-464.
MLA 杨魁,et al."The SARP Family Regulator Txn9 and Two-Component Response Regulator Txn11 are Key Activators for Trioxacarcin Biosynthesis in Streptomyces bottropensis".Curr. Microbiol. 71.4(2015):458-464.
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