SIOC OpenIR  > 生命有机化学国家重点实验室
Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis
Alternative Title硫肽nosiheptide生物合成最后一般碳酰胺化被NosA催化形成的结构研究
Liu SS(刘珊珊)1; Guo H(郭恒)1; Zhang TL(张天龙)1; Han L(韩莉)1; Me PF(么鹏飞)1; Zhang Y(张岩)1; Rong NY(荣乃燕)1; Yu Y(虞沂)1; Lan WX(蓝文贤)1; Wang CX(王春喜)1; Ding JP(丁建平)1; Wang RX(王任小)1; Liu W(刘文)1; Cao CY(曹春阳)1
2015
Source PublicationSci Rep
Volume5Pages:12744
AbstractNosiheptide is a parent compound of thiopeptide family that exhibit potent activities against various bacterial pathogens. Its C-terminal amide formation is catalyzed by NosA, which is an unusual strategy for maturating certain thiopeptides by processing their precursor peptides featuring a serine extension. We here report the crystal structure of truncated NosA(1-111) variant, revealing three key elements, including basic lysine 49 (K49), acidic glutamic acid 101 (E101) and flexible C-terminal loop NosA(112-151), are crucial to the catalytic terminal amide formation in nosiheptide biosynthesis. The side-chain of residue K49 and the C-terminal loop fasten the substrate through hydrogen bonds and hydrophobic interactions. The side-chain of residue E101 enhances nucleophilic attack of H2O to the methyl imine intermediate, leading to C-alpha-N bond cleavage and nosiheptide maturation. The sequence alignment of NosA and its homologs NocA, PbtH, TpdK and BerI, and the enzymatic assay suggest that the mechanistic studies on NosA present an intriguing paradigm about how NosA family members function during thiopeptide biosynthesis.
Subtype论文
Subject Area生命有机化学
DOI10.1038/srep12744
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000359128600001
Citation statistics
Cited Times:9[WOS]   [WOS Record]     [Related Records in WOS]
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39517
Collection生命有机化学国家重点实验室
Corresponding AuthorLiu W(刘文); Cao CY(曹春阳)
Affiliation1.中科院上海有机化学研究所, 生命有机化学国家重点实验室
2.中科院上海有机化学研究所, 生物与化学交叉研究中心
3.中科院上海生命科学研究院生物化学与细胞生物学研究所
Recommended Citation
GB/T 7714
Liu SS,Guo H,Zhang TL,et al. Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis[J]. Sci Rep,2015,5:12744.
APA 刘珊珊.,郭恒.,张天龙.,韩莉.,么鹏飞.,...&曹春阳.(2015).Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis.Sci Rep,5,12744.
MLA 刘珊珊,et al."Structure-based Mechanistic Insights into Terminal Amide Synthase in Nosiheptide-Represented Thiopeptides Biosynthesis".Sci Rep 5(2015):12744.
Files in This Item:
File Name/Size DocType Version Access License
2015404.pdf(1827KB)期刊论文作者接受稿开放获取CC BY-NC-SAApplication Full Text
Related Services
Recommend this item
Bookmark
Usage statistics
Export to Endnote
Google Scholar
Similar articles in Google Scholar
[刘珊珊]'s Articles
[郭恒]'s Articles
[张天龙]'s Articles
Baidu academic
Similar articles in Baidu academic
[刘珊珊]'s Articles
[郭恒]'s Articles
[张天龙]'s Articles
Bing Scholar
Similar articles in Bing Scholar
[刘珊珊]'s Articles
[郭恒]'s Articles
[张天龙]'s Articles
Terms of Use
No data!
Social Bookmark/Share
All comments (0)
No comment.
 

Items in the repository are protected by copyright, with all rights reserved, unless otherwise indicated.