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Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase
Alternative Title三欣卡辛的生物合成揭示了II型聚酮合酶中新的启示单元及复杂的后修饰
Zhang M(张媚)1; Hou XF(侯现锋)1; Qi LH(漆丽华)1; Yin L(殷乐)1; Li Q(李晴)1; Pan HX(潘海学)1; Chen XY(陈新雅)1; Tang GL(唐功利)1
2015
Source PublicationChem. Sci.
Volume6Issue:6Pages:3440-3447
AbstractTrioxacarcins (TXNs) are highly oxygenated, polycyclic aromatic natural products with remarkable biological activity and structural complexity. Evidence from C-13-labelled precursor feeding studies demonstrated that the scaffold was biosynthesized from one unit of L-isoleucine and nine units of malonyl-CoA, which suggested a different starter unit in the biosynthesis. Genetic analysis of the biosynthetic gene cluster revealed 56 genes encoding a type II polyketide synthase (PKS), combined with a large amount of tailoring enzymes. Inactivation of seven post-PKS modification enzymes resulted in the production of a series of new TXN analogues, intermediates, and shunt products, most of which show high anti-cancer activity. Structural elucidation of these new compounds not only helps us to propose the biosynthetic pathway, featuring a type II PKS using a novel starter unit, but also set the stage for further characterization of the enzymatic reactions and combinatorial biosynthesis.
Subtype论文
Subject Area生命有机化学
DOI10.1039/c5sc00116a
URL查看原文
Indexed BySCI
Language英语
WOS IDWOS:000354815600022
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Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/39507
Collection生命有机化学国家重点实验室
Corresponding AuthorTang GL(唐功利)
Affiliation1.中科院上海有机化学研究所, 生命有机化学国家重点实验室
2.上海生物制造技术协同创新中心
Recommended Citation
GB/T 7714
Zhang M,Hou XF,Qi LH,et al. Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase[J]. Chem. Sci.,2015,6(6):3440-3447.
APA 张媚.,侯现锋.,漆丽华.,殷乐.,李晴.,...&唐功利.(2015).Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase.Chem. Sci.,6(6),3440-3447.
MLA 张媚,et al."Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase".Chem. Sci. 6.6(2015):3440-3447.
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