中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 生命有机化学国家重点实验室  > 期刊论文
学科主题: 生命有机化学
题名: Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase
其他题名: 三欣卡辛的生物合成揭示了II型聚酮合酶中新的启示单元及复杂的后修饰
作者: Zhang M(张媚)1; Hou XF(侯现锋)1; Qi LH(漆丽华)1; Yin L(殷乐)1; Li Q(李晴)1; Pan HX(潘海学)1; Chen XY(陈新雅)1; Tang GL(唐功利)1
通讯作者: 唐功利
刊名: Chem. Sci.
发表日期: 2015
DOI: 10.1039/c5sc00116a
卷: 6, 期:6, 页:3440-3447
收录类别: SCI
文章类型: 论文
英文摘要: Trioxacarcins (TXNs) are highly oxygenated, polycyclic aromatic natural products with remarkable biological activity and structural complexity. Evidence from C-13-labelled precursor feeding studies demonstrated that the scaffold was biosynthesized from one unit of L-isoleucine and nine units of malonyl-CoA, which suggested a different starter unit in the biosynthesis. Genetic analysis of the biosynthetic gene cluster revealed 56 genes encoding a type II polyketide synthase (PKS), combined with a large amount of tailoring enzymes. Inactivation of seven post-PKS modification enzymes resulted in the production of a series of new TXN analogues, intermediates, and shunt products, most of which show high anti-cancer activity. Structural elucidation of these new compounds not only helps us to propose the biosynthetic pathway, featuring a type II PKS using a novel starter unit, but also set the stage for further characterization of the enzymatic reactions and combinatorial biosynthesis.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000354815600022
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/39507
Appears in Collections:生命有机化学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2015293.pdf(941KB)期刊论文作者接受稿开放获取View Download

作者单位: 1.中科院上海有机化学研究所, 生命有机化学国家重点实验室
2.上海生物制造技术协同创新中心

Recommended Citation:
Zhang M,Hou XF,Qi LH,et al. Biosynthesis of trioxacarcin revealing a different starter unit and complex tailoring steps for type II polyketide synthase[J]. Chem. Sci.,2015,6(6):3440-3447.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[张媚]'s Articles
[侯现锋]'s Articles
[漆丽华]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[张媚]‘s Articles
[侯现锋]‘s Articles
[漆丽华]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2015293.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace