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The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B
Alternative TitleThe PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B
Zhang Y(张岩)1; YANG HUIRONG1; GUO XUE1; RONG NAIYAN1; SONG YUJIAO1; XU YOUWEI1; LAN WENXIAN1; ZHANG XU1; LIU MAILI1; XU YANHUI1; Cao CY(曹春阳)1
2014
Source PublicationProtein Cell
Volume5Issue:11Pages:837-850
AbstractKDM5B is a histone H3K4me2/3 demethylase. The PHD1 domain of KDM5B is critical for demethylation, but the mechanism underlying the action of this domain is unclear. In this paper, we observed that PHD1(KDM5B) interacts with unmethylated H3K4me0. Our NMR structure of PHD1(KDM5B) in complex with H3K4me0 revealed that the binding mode is slightly different from that of other reported PHD fingers. The disruption of this interaction by double mutations on the residues in the interface (L325A/D328A) decreases the H3K4me2/3 demethylation activity of KDM5B in cells by approximately 50% and increases the transcriptional repression of tumor suppressor genes by approximately twofold. These findings imply that PHD1(KDM5B) may help maintain KDM5B at target genes to mediate the demethylation activities of KDM5B.
Subject Area生命有机化学
DOI10.1007/s13238-014-0078-4
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Indexed BySCI
Language英语
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Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/38962
Collection生命有机化学国家重点实验室
Corresponding AuthorXU YANHUI; Cao CY(曹春阳)
Affiliation1.中科院上海有机化学研究所
2.复旦大学
3.中科院武汉物理与数学研究所
Recommended Citation
GB/T 7714
Zhang Y,YANG HUIRONG,GUO XUE,et al. The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B[J]. Protein Cell,2014,5(11):837-850.
APA 张岩.,YANG HUIRONG.,GUO XUE.,RONG NAIYAN.,SONG YUJIAO.,...&曹春阳.(2014).The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B.Protein Cell,5(11),837-850.
MLA 张岩,et al."The PHD1 finger of KDM5B recognizes unmodified H3K4 during the demethylation of histone H3K4me2/3 by KDM5B".Protein Cell 5.11(2014):837-850.
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