An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis

doi:10.1002/anie.201406602

	An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis
其他题名	聚酮起始单元生物合成中一个新的脱水酶作用于甘油底物及酮基还原酶立体选择性还原a-酮基
	He HY(贺海燕)1; Yuan H(袁华)1; Tang MC(唐满成)1; Tang GL(唐功利)1
	2014
发表期刊	Angew. Chem.-Int. Edit.
卷号	53 期号:42 页码:11315-11319
摘要	Polyketide synthases (PKSs) usually employ a ketoreductase (KR) to catalyze the reduction of a -keto group, followed by a dehydratase (DH) that drives the dehydration to form a double bond between the - and -carbon atoms. Herein, a DH-KR involved in FR901464 biosynthesis was characterized: DH* acts on glyceryl-S-acyl carrier protein (ACP) to yield ACP-linked pyruvate; subsequently KR* reduces -ketone that yields L-lactyl-S-ACP as starter unit for polyketide biosynthesis. Genetic and biochemical evidence was found to support a similar pathway that is involved in the biosynthesis of lankacidins. These results not only identified new PKS domains acting on different substrates, but also provided additional options for engineering the PKS starter pathway or biocatalysis.
学科领域	生命有机化学
DOI	10.1002/anie.201406602
URL	查看原文
收录类别	SCI
语种	英语
引用统计	被引频次：6[WOS] [WOS记录] [WOS相关记录]
文献类型	期刊论文
条目标识符	http://ir.sioc.ac.cn/handle/331003/38960
专题	生命有机化学国家重点实验室
通讯作者	Tang GL(唐功利)
作者单位	1.中科院上海有机化学研究所 2.上海生物制造技术协同创新中心
推荐引用方式 GB/T 7714	He HY,Yuan H,Tang MC,et al. An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis[J]. Angew. Chem.-Int. Edit.,2014,53(42):11315-11319.
APA	贺海燕,袁华,唐满成,&唐功利.(2014).An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis.Angew. Chem.-Int. Edit.,53(42),11315-11319.
MLA	贺海燕,et al."An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis".Angew. Chem.-Int. Edit. 53.42(2014):11315-11319.

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