| An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis | |
| Alternative Title | 聚酮起始单元生物合成中一个新的脱水酶作用于甘油底物及酮基还原酶立体选择性还原a-酮基 |
| He HY(贺海燕)1; Yuan H(袁华)1; Tang MC(唐满成)1; Tang GL(唐功利)1 | |
| 2014 | |
| Source Publication | Angew. Chem.-Int. Edit.
 |
| Volume | 53Issue:42Pages:11315-11319 |
| Abstract | Polyketide synthases (PKSs) usually employ a ketoreductase (KR) to catalyze the reduction of a -keto group, followed by a dehydratase (DH) that drives the dehydration to form a double bond between the - and -carbon atoms. Herein, a DH*-KR* involved in FR901464 biosynthesis was characterized: DH* acts on glyceryl-S-acyl carrier protein (ACP) to yield ACP-linked pyruvate; subsequently KR* reduces -ketone that yields L-lactyl-S-ACP as starter unit for polyketide biosynthesis. Genetic and biochemical evidence was found to support a similar pathway that is involved in the biosynthesis of lankacidins. These results not only identified new PKS domains acting on different substrates, but also provided additional options for engineering the PKS starter pathway or biocatalysis. |
| Subject Area | 生命有机化学 |
| DOI | 10.1002/anie.201406602 |
| URL | 查看原文 |
| Indexed By | SCI |
| Language | 英语 |
| Citation statistics | |
| Document Type | 期刊论文 |
| Identifier | http://ir.sioc.ac.cn/handle/331003/38960 |
| Collection | 生命有机化学国家重点实验室 |
| Corresponding Author | Tang GL(唐功利) |
| Affiliation | 1.中科院上海有机化学研究所 2.上海生物制造技术协同创新中心 |
| Recommended Citation GB/T 7714 | He HY,Yuan H,Tang MC,et al. An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis[J]. Angew. Chem.-Int. Edit.,2014,53(42):11315-11319. |
| APA | 贺海燕,袁华,唐满成,&唐功利.(2014).An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis.Angew. Chem.-Int. Edit.,53(42),11315-11319. |
| MLA | 贺海燕,et al."An Unusual Dehydratase Acting on Glycerate and a Ketoreducatse Stereoselectively Reducing alpha-Ketone in Polyketide Starter Unit Biosynthesis".Angew. Chem.-Int. Edit. 53.42(2014):11315-11319. |
| Files in This Item: | ||||||
| File Name/Size | DocType | Version | Access | License | ||
| 2014501.pdf（678KB） | 期刊论文 | 作者接受稿 | 开放获取 | CC BY-NC-SA | Application Full Text | |
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