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Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain
Alternative Title阿维菌素高产菌中AvaR作为一个自调控受体负调控阿维菌素产生
Wang JB(王健博); Zhang F(张凤); Pu JY(蒲津越); Zhao J(赵娟); Zhao QF(赵群飞); Tang GL(唐功利)
2014
Source PublicationBiotechnol. Lett.
Volume36Issue:4Pages:813-819
AbstractMany gamma-butyrolactone-autoregulator receptors control the production of secondary metabolites in Streptomyces spp. Hence, AvaR1, an autoregulator receptor protein in Streptomyces avermitilis, was characterized as a negative regulator of avermectin (Ave) production. Deletion of AvaR1 in a high-producing strain increased production of Ave B1a approx. 1.75 times (similar to 700 mu g/ml) compared with the parent strain. Semi-quantitative RT-PCR and electrophoretic mobility shift assays revealed that AvaR1 regulates the biosynthesis of Ave but not through the aveR pathway-specific regulatory gene. A special signaling molecule, avenolide, increased production of Ave. This study has refined our understanding of how avenolide regulates the production of Aves which is promising for developing new methods to improve the production of antibiotics in industrial strains.
Subject Area生命有机化学
DOI10.1007/s10529-013-1416-y
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Indexed BySCI
Language英语
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Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/38936
Collection生命有机化学国家重点实验室
Corresponding AuthorTang GL(唐功利)
Affiliation中科院上海有机化学研究所
Recommended Citation
GB/T 7714
Wang JB,Zhang F,Pu JY,et al. Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain[J]. Biotechnol. Lett.,2014,36(4):813-819.
APA 王健博,张凤,蒲津越,赵娟,赵群飞,&唐功利.(2014).Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain.Biotechnol. Lett.,36(4),813-819.
MLA 王健博,et al."Characterization of AvaR1, an autoregulator receptor that negatively controls avermectins production in a high avermectin-producing strain".Biotechnol. Lett. 36.4(2014):813-819.
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