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学科主题: 高分子化学
题名: Cholesterol-based cationic lipids for gene delivery: Contribution of molecular structure factors to physico-chemical and biological properties
其他题名: 基于甾体化合物的脂质体基因载体: 分子结构因子对物理化学性能和生物性能的贡献
作者: Sheng RL(盛瑞隆); Luo T(罗挺); Li H(李慧); Sun JJ(孙景景); Wang Z(王昭); Cao AM(曹阿民)
通讯作者: 曹阿民
刊名: Colloid Surf. B-Biointerfaces
发表日期: 2014
DOI: 10.1016/j.colsurfb.2013.12.039
卷: 116, 页:32-40
收录类别: SCI
英文摘要: In this work, we prepared a series of cholesterol-based cationic (Cho-cat) lipids bearing cholesterol hydrophobe, natural amino acid headgroups (lysine/histidine) and linkage (carbonate ester/ether) bonds. In which, the natural amino acid headgroups made dominant contribution to their physico-chemical and biological properties. Among the lipids, the L-lysine headgroup bearing lipids (Cho-es/et-Lys) showed higher pDNA binding affinity and were able to form larger sized and higher surface charged lipoplexes than that of L-histidine headgroup bearing lipids (Cho-es/et-His), they also demonstrated higher transfection efficacy and higher membrane disruption capacities than that of their L-histidine headgroup bearing counterparts. However, compared to the contributions of the headgroups, the (carbonate ester/ether) linkage bonds showed much less affects. Besides, it could be noted that, Cho-es/et-Lys lipids exhibited very high luciferase gene transfection efficiency that almost reached the transfection level of "gold standard" bPEI-25k, made them potential transfection reagents for practical application. Moreover, the results facilitated the understanding for the structure activity relationship of the cholesterol-based cationic lipids, and also paved a simple and efficient way for achieving high transfection efficiency by modification of suitable headgroups on lipid gene carriers. (C) 2014 Elsevier B.V. All rights reserved.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/38892
Appears in Collections:高分子材料研究室_期刊论文

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作者单位: 中科院上海有机化学研究所

Recommended Citation:
Sheng RL,Luo T,Li H,et al. Cholesterol-based cationic lipids for gene delivery: Contribution of molecular structure factors to physico-chemical and biological properties[J]. Colloid Surf. B-Biointerfaces,2014,116:32-40.
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