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N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues
其他题名氮烷基吡啶季铵化与高效液相色谱串联质谱结合用于高灵敏的分析人甲状腺肿瘤组织中的脂肪醇
Cao YJ(曹燕静)1; Guan Q(官青)1; Sun TQ(孙团起)1; Wang H(汪航)1; Leng JP(冷嘉鹏)1; Guo YL(郭寅龙)1
2014
发表期刊Anal. Chim. Acta
卷号849页码:19-26
摘要A highly sensitive method was developed for the identification and quantification of fatty alcohols in biological tissues. In the presence of pyridine-d(0) and triflic anhydride (Tf2O), fatty alcohols were converted into permanently charged N-alkylpyridinium ions. Stable isotope-labeled derivatives were generated by pyridine-d(5) and added as internal standard (IS). The mixture was analyzed by liquid chromatography coupled to positive electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This method was optimized and validated in terms of reaction time, derivatization efficiency, stability, desalting, and ion suppression effect. Besides, fatty alcohols exhibited good linear relationship (r2 > 0.993) over the concentration range of 10 ng mL(1)-1 mu g mL(1). The limits of detection (LODs) were lowered from previously reported 0.1 ng mL(1) to 0.25 pg mL(1). Precision (RSD% < 15.6%), accuracy (93.0-107.2%), matrix effect, and recovery (in thyroid tissues) were validated as well. Finally, this method was applied for the analysis of ten even carbon-numbered fatty alcohols (C8-C24) in human thyroid procedure achieved a sufficient enhancement of sensitivity. Furthermore, the exact concentrations of total (free and esterfied) fatty alcohols in human thyroid tissues were measured.
学科领域分析化学
DOI10.1016/j.aca.2014.08.027
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收录类别SCI
语种英语
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文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/38592
专题分析化学研究室
通讯作者Sun TQ(孙团起); Guo YL(郭寅龙)
作者单位1.中科院上海有机化学研究所
2.复旦大学
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GB/T 7714
Cao YJ,Guan Q,Sun TQ,et al. N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues[J]. Anal. Chim. Acta,2014,849:19-26.
APA 曹燕静,官青,孙团起,汪航,冷嘉鹏,&郭寅龙.(2014).N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues.Anal. Chim. Acta,849,19-26.
MLA 曹燕静,et al."N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues".Anal. Chim. Acta 849(2014):19-26.
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