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学科主题: 分析化学
题名: N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues
其他题名: 氮烷基吡啶季铵化与高效液相色谱串联质谱结合用于高灵敏的分析人甲状腺肿瘤组织中的脂肪醇
作者: Cao YJ(曹燕静)1; Guan Q(官青)1; Sun TQ(孙团起)1; Wang H(汪航)1; Leng JP(冷嘉鹏)1; Guo YL(郭寅龙)1
通讯作者: 孙团起 ; 郭寅龙
刊名: Anal. Chim. Acta
发表日期: 2014
DOI: 10.1016/j.aca.2014.08.027
卷: 849, 页:19-26
收录类别: SCI
英文摘要: A highly sensitive method was developed for the identification and quantification of fatty alcohols in biological tissues. In the presence of pyridine-d(0) and triflic anhydride (Tf2O), fatty alcohols were converted into permanently charged N-alkylpyridinium ions. Stable isotope-labeled derivatives were generated by pyridine-d(5) and added as internal standard (IS). The mixture was analyzed by liquid chromatography coupled to positive electrospray ionization tandem mass spectrometry (LC-ESI-MS/MS). This method was optimized and validated in terms of reaction time, derivatization efficiency, stability, desalting, and ion suppression effect. Besides, fatty alcohols exhibited good linear relationship (r2 > 0.993) over the concentration range of 10 ng mL(1)-1 mu g mL(1). The limits of detection (LODs) were lowered from previously reported 0.1 ng mL(1) to 0.25 pg mL(1). Precision (RSD% < 15.6%), accuracy (93.0-107.2%), matrix effect, and recovery (in thyroid tissues) were validated as well. Finally, this method was applied for the analysis of ten even carbon-numbered fatty alcohols (C8-C24) in human thyroid procedure achieved a sufficient enhancement of sensitivity. Furthermore, the exact concentrations of total (free and esterfied) fatty alcohols in human thyroid tissues were measured.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/38592
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 1.中科院上海有机化学研究所
2.复旦大学

Recommended Citation:
Cao YJ,Guan Q,Sun TQ,et al. N-alkylpyridinium quaternization combined with liquid chromatography-electrospray ionization-tandem mass spectrometry: A highly sensitive method to quantify fatty alcohols in thyroid tissues[J]. Anal. Chim. Acta,2014,849:19-26.
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