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学科主题: 分析化学
题名: Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry
其他题名: 亲和整体柱结合液相色谱-串联质谱用于研究药物结合蛋白谱
作者: Zhang XP(张雪佩)1; Wang TD(汪彤丹)1; Zhang HZ(张含智)1; Han B(韩冰)1; Wang LS(王立顺)1; Kang JW(康经武)1
通讯作者: 康经武
刊名: J. Chromatogr. A
发表日期: 2014
DOI: 10.1016/j.chroma.2014.07.020
卷: 1359, 页:84-90
收录类别: SCI
英文摘要: A new approach for proteome-wide profiling drug binding proteins by using monolithic capillary affinity chromatography in combination with HPLC-MS/MS is reported. Two immunosuppresive drugs, namely FK506 and cyclosporin A, were utilized as the experimental models for proof-of-concept. The monolithic capillary affinity columns were prepared through a single-step copolymerization of the drug derivatives with glycidyl methacrylate and ethylene dimethacrylate. The capillary chromatography with the affinity monolithic column facilitates the purification of the drug binding proteins from the cell lysate. By combining the capillary affinity column purification and the shot-gun proteomic analysis, totally 33 FK506- and 32 CsA-binding proteins including all the literature reported target proteins of these two drugs were identified. Among them, two proteins, namely voltage-dependent anion-selective channel protein 1 and serine/threonine-protein phosphatase PGAM5 were verified by using the recombinant proteins. The result supports that the monolithic capillary affinity chromatography is likely to become a valuable tool for profiling of binding proteins of small molecular drugs as well as bioactive compounds. (C) 2014 Elsevier B.V. All rights reserved.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/38588
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 1.中科院上海有机化学研究所
2.上海交通大学
3.上海科技大学

Recommended Citation:
Zhang XP,Wang TD,Zhang HZ,et al. Profiling of drug binding proteins by monolithic affinity chromatography in combination with liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2014,1359:84-90.
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