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Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry
其他题名Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry
Wang TD(汪彤丹); Zhang QQ(张倩倩); Zhang YM(张艳梅); Kang JW(康经武)
2014
发表期刊J. Chromatogr. A
卷号1337页码:188-193
摘要We report a capillary electrophoresis method in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for screening of protein kinase inhibitors (PKIs) in natural extracts. Protein kinase A (PKA), substrate 5-carboxyfluorescein-labeled kemptide (CLK) and inhibitor H-89 were employed for the method development and validation. Enzymatic inhibition assay was performed with electrophoretically mediated microanalysis technique. Once the bioactivity of a natural extract was confirmed, an assay-guided isolation and structure elucidation using LC-MS/MS were accomplished to identify the compounds which are responsible for the observed bioactivity. Totally 33 natural extracts were screened with the method, and baicalin in the extract of Radix Scutellariae was identified to be a new PKI of PICA. This result demonstrated the practical applicability of our method in screening of PKIs from natural products. (C) 2014 Elsevier B.V. All rights reserved.
学科领域分析化学
DOI10.1016/j.chroma.2014.02.039
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收录类别SCI
语种英语
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被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/38586
专题分析化学研究室
通讯作者Kang JW(康经武)
作者单位中科院上海有机化学研究所
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GB/T 7714
Wang TD,Zhang QQ,Zhang YM,et al. Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2014,1337:188-193.
APA 汪彤丹,张倩倩,张艳梅,&康经武.(2014).Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry.J. Chromatogr. A,1337,188-193.
MLA 汪彤丹,et al."Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry".J. Chromatogr. A 1337(2014):188-193.
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