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学科主题: 分析化学
题名: Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry
其他题名: Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry
作者: Wang TD(汪彤丹); Zhang QQ(张倩倩); Zhang YM(张艳梅); Kang JW(康经武)
通讯作者: 康经武
刊名: J. Chromatogr. A
发表日期: 2014
DOI: 10.1016/j.chroma.2014.02.039
卷: 1337, 页:188-193
收录类别: SCI
英文摘要: We report a capillary electrophoresis method in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for screening of protein kinase inhibitors (PKIs) in natural extracts. Protein kinase A (PKA), substrate 5-carboxyfluorescein-labeled kemptide (CLK) and inhibitor H-89 were employed for the method development and validation. Enzymatic inhibition assay was performed with electrophoretically mediated microanalysis technique. Once the bioactivity of a natural extract was confirmed, an assay-guided isolation and structure elucidation using LC-MS/MS were accomplished to identify the compounds which are responsible for the observed bioactivity. Totally 33 natural extracts were screened with the method, and baicalin in the extract of Radix Scutellariae was identified to be a new PKI of PICA. This result demonstrated the practical applicability of our method in screening of PKIs from natural products. (C) 2014 Elsevier B.V. All rights reserved.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/38586
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 中科院上海有机化学研究所

Recommended Citation:
Wang TD,Zhang QQ,Zhang YM,et al. Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2014,1337:188-193.
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