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Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry
Alternative TitleScreening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry
Wang TD(汪彤丹); Zhang QQ(张倩倩); Zhang YM(张艳梅); Kang JW(康经武)
2014
Source PublicationJ. Chromatogr. A
Volume1337Pages:188-193
AbstractWe report a capillary electrophoresis method in conjunction with liquid chromatography-tandem mass spectrometry (LC-MS/MS) for screening of protein kinase inhibitors (PKIs) in natural extracts. Protein kinase A (PKA), substrate 5-carboxyfluorescein-labeled kemptide (CLK) and inhibitor H-89 were employed for the method development and validation. Enzymatic inhibition assay was performed with electrophoretically mediated microanalysis technique. Once the bioactivity of a natural extract was confirmed, an assay-guided isolation and structure elucidation using LC-MS/MS were accomplished to identify the compounds which are responsible for the observed bioactivity. Totally 33 natural extracts were screened with the method, and baicalin in the extract of Radix Scutellariae was identified to be a new PKI of PICA. This result demonstrated the practical applicability of our method in screening of PKIs from natural products. (C) 2014 Elsevier B.V. All rights reserved.
Subject Area分析化学
DOI10.1016/j.chroma.2014.02.039
URL查看原文
Indexed BySCI
Language英语
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Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/38586
Collection分析化学研究室
Corresponding AuthorKang JW(康经武)
Affiliation中科院上海有机化学研究所
Recommended Citation
GB/T 7714
Wang TD,Zhang QQ,Zhang YM,et al. Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry[J]. J. Chromatogr. A,2014,1337:188-193.
APA 汪彤丹,张倩倩,张艳梅,&康经武.(2014).Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry.J. Chromatogr. A,1337,188-193.
MLA 汪彤丹,et al."Screening of protein kinase inhibitors in natural extracts by capillary electrophoresis combined with liquid chromatography-tandem mass spectrometry".J. Chromatogr. A 1337(2014):188-193.
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