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学科主题: 分析化学
题名: 基于酶抑制原理的电喷雾质谱法快速筛查蔬菜中氨基甲酸酯类农药残留
其他题名: Rapid Screening of Carbamates Pesticide Residues in Vegetables by Electrospray Ionization Mass Spectrometry Based on Principle of Enzyme Inhibition
作者: 罗辉泰1; 黄晓兰1; 吴惠勤1; 张立1; 郭寅龙1; 朱志鑫1; 黄芳1; 林晓珊1
通讯作者: 黄晓兰
刊名: 分析化学
发表日期: 2014
卷: 42, 期:11, 页:1561-1567
收录类别: SCI
英文摘要: A rapid screening method for the determination of carbamate pesticides (CBPs) residues in vegetables by measuring acetylcholinesterase (AChE) inhibition rate using electrospray ionization mass spectrometry (ESI-MS) has been established. After pretreatment by QuEChERS method, sample solution reacts with AChE using acetylthiocholine as substrate. AChE inhibition rate was calculated by determination of the conversion of substrate to product (thiocholine) using ESI. MS. The temperature, time and concentration conditions of enzymatic reactions have been optimized. The relationship between the concentration of 10 kinds of common CBPs and AChE inhibition rate was researched. Matrix effects of real vegetables were studied. The limit of detection (LOD), which was measured by 3 times of enzyme inhibition rate of pesticide-free vegetable samples, was 0.01-0. 05 mg/kg. The results showed that the method was better than the current national standard method of china for rapid screening of pesticide residues and fully meet the requirements of maximum residue limits(MRL) for pesticides in food of national food safety standard. False positive results were avoided effectively due to its good ability of resistance matrix interference. The reliability was proved by analyzing vegetables with liquid chromatography-tandem mass spectrometry. The method is simple, rapid, sensitive, reliable, and can be used for the rapid, high-throughput screening of CBPs in vegetables.
语种: 中文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/38585
Appears in Collections:分析化学研究室_期刊论文

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作者单位: 1.中国广州分析测试中心
2.中科院上海有机化学研究所

Recommended Citation:
罗辉泰,黄晓兰,吴惠勤,等. 基于酶抑制原理的电喷雾质谱法快速筛查蔬菜中氨基甲酸酯类农药残留[J]. 分析化学,2014,42(11):1561-1567.
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