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Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum
其他题名Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum
Deng WH(邓文华); Cheng JY(程佳懿); Li TH(李通化); Kang JW(康经武)
2010
发表期刊Chin. J. Chem.
ISSN1001-604X
卷号28期号:6页码:988-992
摘要A method for determination of intracellular acyl-coenzyme A esters in Clostridium acetobutylicum (CA) by high performance liquid chromatography (HPLC) was developed and validated. In our experiment, two important intermediates acyl-coenzyme A esters including acetyl-CoA, butyryl-CoA could be baseline separated on a Zobax-C18 column with mobile phase composed of the acetonitrile and phosphate buffer (pH 5.0). Samples treated with freeze-thaw and protein precipitated by addition of trichloroacetic acid could be directly injected for determination of acetyl-CoA, butyryl-CoA with a recovery higher than 73%. With this method, the metabolite profiling of the acyl-coenzyme A esters of CA was obtained. The comparison of the metabolite profiling between the model strain ATCC 824 and a mutant strain EA 2018, which produces higher butanol than the former was performed. Some useful information was concluded for understanding the mechanism of CA for selectively producing the solvent.
学科领域分析化学
部门归属中国科学院上海有机化学研究所; 同济大学
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收录类别SCI
语种英语
WOS记录号WOS:000279376200020
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文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28683
专题分析化学研究室
通讯作者Kang JW(康经武)
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GB/T 7714
Deng WH,Cheng JY,Li TH,et al. Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum[J]. Chin. J. Chem.,2010,28(6):988-992.
APA 邓文华,程佳懿,李通化,&康经武.(2010).Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum.Chin. J. Chem.,28(6),988-992.
MLA 邓文华,et al."Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum".Chin. J. Chem. 28.6(2010):988-992.
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