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学科主题: 分析化学
题名: Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum
其他题名: Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum
作者: Deng WH(邓文华) ; Cheng JY(程佳懿) ; Li TH(李通化) ; Kang JW(康经武)
通讯作者: 康经武
刊名: Chin. J. Chem.
发表日期: 2010-01-01
卷: 28, 期:6, 页:988-992
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所; 同济大学
英文摘要: A method for determination of intracellular acyl-coenzyme A esters in Clostridium acetobutylicum (CA) by high performance liquid chromatography (HPLC) was developed and validated. In our experiment, two important intermediates acyl-coenzyme A esters including acetyl-CoA, butyryl-CoA could be baseline separated on a Zobax-C18 column with mobile phase composed of the acetonitrile and phosphate buffer (pH 5.0). Samples treated with freeze-thaw and protein precipitated by addition of trichloroacetic acid could be directly injected for determination of acetyl-CoA, butyryl-CoA with a recovery higher than 73%. With this method, the metabolite profiling of the acyl-coenzyme A esters of CA was obtained. The comparison of the metabolite profiling between the model strain ATCC 824 and a mutant strain EA 2018, which produces higher butanol than the former was performed. Some useful information was concluded for understanding the mechanism of CA for selectively producing the solvent.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000279376200020
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28683
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Deng WH,Cheng JY,Li TH,et al. Determination of Intracellular Concentration of Acyl-Coenzyme A Esters for Metabolic Profiling Clostridium acetobutylicum[J]. Chin. J. Chem.,2010,28(6):988-992.
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