中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 分析化学研究室  > 期刊论文
学科主题: 分析化学
题名: Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers
其他题名: 聚电解质动态涂层用于亲和毛细管电泳前沿分析方法
作者: Liu C(刘超) ; Kang JW(康经武)
通讯作者: 康经武
刊名: J. Chromatogr. A
发表日期: 2012
卷: 1238, 页:146-151
收录类别: SCI
部门归属: 中科院上海有机化学研究所
英文摘要: An improved CE-FA method using a polyelectrolyte multilayer (PEML) coated capillary is described. The coating was simply created by alternatively flushing the capillary with positively charged polyelectrolyte polybrene (HDB) and negatively charged polyelectrolyte dextran sulfate (DS). Human serum albumin (HSA) and 6 drugs were selected as an experimental model to evaluate such an improvement. The PEML coating was demonstrated to effectively reduce the protein adsorption on the capillary wall. Compared with the uncoated capillary, the repeatability (RSD%) of the EOF mobility and the plateau height of free drug were improved significantly from 3.7% to 0.51% and from 0.61% to 0.34%, respectively. No interference of the coating on the measurement of protein-drug binding parameters was observed. (C) 2012 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000303843000018
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28669
Appears in Collections:分析化学研究室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2012094.pdf(563KB)----开放获取--View Download

Recommended Citation:
Liu C,Kang JW. Improved capillary electrophoresis frontal analysis by dynamically coating the capillary with polyelectrolyte multilayers[J]. J. Chromatogr. A,2012,1238:146-151.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[刘超]'s Articles
[康经武]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[刘超]‘s Articles
[康经武]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2012094.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace