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学科主题: 分析化学
题名: Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry
其他题名: 氮端同位素标记技术结合纳流液相/基质辅助激光解析离子化质谱分析人体血清中不同表达的低质量多肽
作者: Leng JP(冷嘉鹏) ; Zhu D(朱东) ; Wu DJ(武多娇) ; Zhu TY(朱同玉) ; Zhao NW(赵宁伟) ; Guo YL(郭寅龙)
通讯作者: 朱同玉 ; 郭寅龙
刊名: Rapid Commun. Mass Spectrom.
发表日期: 2012
卷: 26, 期:21, 页:2555-2562
收录类别: SCI
部门归属: 中科院上海有机化学研究所; 复旦大学附属中山医院; Shimadzu Global COE Applicat & Tech Dev
英文摘要: RATIONALE Peptidomics analysis of human serum is challenging due to the low abundance of serum peptides and interference from the complex matrix. This study analyzed the differentially expressed (DE) low molecular weight peptides in human serum integrating a DMPITC-based N-terminal isotope labeling technique with nano-liquid chromatography and matrix-assisted laser desorption/ionization mass spectrometry (nano-LC/MALDI-MS). METHODS The workflow introduced a [d6]-4,6-dimethoxypyrimidine-2-isothiocyanate (DMPITC)-labeled mixture of aliquots from test samples as the internal standard. The spiked [d0]-DMPITC-labeled samples were separated by nano-LC then spotted on the MALDI target. Both quantitative and qualitative studies for serum peptides were achieved based on the isotope-labeled peaks. RESULTS The DMPITC labeling technique combined with nano-LC/MALDI-MS not only minimized the errors in peptide quantitation, but also allowed convenient recognition of the labeled peptides due to the 6?Da mass difference. The data showed that the entire research procedure as well as the subsequent data analysis method were effective, reproducible, and sensitive for the analysis of DE serum peptides. CONCLUSIONS This study successfully established a research model for DE serum peptides using DMPITC-based N-terminal isotope labeling and nano-LC/MALDI-MS. Application of the DMPITC-based N-terminal labeling technique is expected to provide a promising tool for the investigation of peptides in vivo, especially for the analysis of DE peptides under different biological conditions. Copyright (C) 2012 John Wiley & Sons, Ltd.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000309182200009
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28659
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Leng JP,Zhu D,Wu DJ,et al. Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry[J]. Rapid Commun. Mass Spectrom.,2012,26(21):2555-2562.
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