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Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry
其他题名氮端同位素标记技术结合纳流液相/基质辅助激光解析离子化质谱分析人体血清中不同表达的低质量多肽
Leng JP(冷嘉鹏); Zhu D(朱东); Wu DJ(武多娇); Zhu TY(朱同玉); Zhao NW(赵宁伟); Guo YL(郭寅龙)
2012
发表期刊Rapid Commun. Mass Spectrom.
ISSN0951-4198
卷号26期号:21页码:2555-2562
摘要RATIONALE Peptidomics analysis of human serum is challenging due to the low abundance of serum peptides and interference from the complex matrix. This study analyzed the differentially expressed (DE) low molecular weight peptides in human serum integrating a DMPITC-based N-terminal isotope labeling technique with nano-liquid chromatography and matrix-assisted laser desorption/ionization mass spectrometry (nano-LC/MALDI-MS). METHODS The workflow introduced a [d6]-4,6-dimethoxypyrimidine-2-isothiocyanate (DMPITC)-labeled mixture of aliquots from test samples as the internal standard. The spiked [d0]-DMPITC-labeled samples were separated by nano-LC then spotted on the MALDI target. Both quantitative and qualitative studies for serum peptides were achieved based on the isotope-labeled peaks. RESULTS The DMPITC labeling technique combined with nano-LC/MALDI-MS not only minimized the errors in peptide quantitation, but also allowed convenient recognition of the labeled peptides due to the 6?Da mass difference. The data showed that the entire research procedure as well as the subsequent data analysis method were effective, reproducible, and sensitive for the analysis of DE serum peptides. CONCLUSIONS This study successfully established a research model for DE serum peptides using DMPITC-based N-terminal isotope labeling and nano-LC/MALDI-MS. Application of the DMPITC-based N-terminal labeling technique is expected to provide a promising tool for the investigation of peptides in vivo, especially for the analysis of DE peptides under different biological conditions. Copyright (C) 2012 John Wiley & Sons, Ltd.
学科领域分析化学
部门归属中科院上海有机化学研究所; 复旦大学附属中山医院; Shimadzu Global COE Applicat & Tech Dev
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收录类别SCI
语种英语
WOS记录号WOS:000309182200009
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文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28659
专题分析化学研究室
通讯作者Zhu TY(朱同玉); Guo YL(郭寅龙)
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GB/T 7714
Leng JP,Zhu D,Wu DJ,et al. Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry[J]. Rapid Commun. Mass Spectrom.,2012,26(21):2555-2562.
APA 冷嘉鹏,朱东,武多娇,朱同玉,赵宁伟,&郭寅龙.(2012).Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry.Rapid Commun. Mass Spectrom.,26(21),2555-2562.
MLA 冷嘉鹏,et al."Analysis of the differentially expressed low molecular weight peptides in human serum via an N-terminal isotope labeling technique combining nano-liquid chromatography/matrix-assisted laser desorption/ionization mass spectrometry".Rapid Commun. Mass Spectrom. 26.21(2012):2555-2562.
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