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Rapid Detection of Irreversible Acetylcholineasterase Inhibitor by Mass Spectrometry Assay
其他题名质谱法快速检测不可逆乙酰胆碱酯酶抑制剂
Cai TT(蔡婷婷); Zhang L(张立); Wang R(汪蓉); Liang C(梁晨); Zhao WS(赵武生); Fu DF(傅得锋); Zhang YR(张玉荣); Guo YL(郭寅龙)
2012
发表期刊Chin. J. Chem.
ISSN1001-604X
卷号30期号:8页码:1788-1792
摘要Here we developed a rapid method to detect acetylcholinesterase (AChE) activity by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) for screening irreversible AChE inhibitors. Due to its good salt-tolerance and low sample consumption, MALDI-FTMS could facilitate rapid detection, especially detection in real application. AChE activity was determined through calculating abundance of substrate and product in mass spectrometry. By this approach, we investigated the relation of organophosphorous (OP) concentrations and AChE inhibition. Shown in different inhibition curves from different OP pesticides, enzyme inhibitions still kept good correlation with concentration of OPs. Finally, this AChE-inhibited method was applied to screen whole bloods of four decedents and discuss their death reason. In contrast to healthy persons, three of decedents showed low AChE activity, and probably died for irreversible AChE inhibitors. Through the following detecting in GC-MS/MS, the possible death reason of these three decedents was confirmed, and another decedent actually died for sumicidin, a non-AChE inhibitor. It demonstrated that screening irreversible AChE inhibitors by detecting enzyme activity in MALDI-FTMS provided fast and accurate analysis results and excluded another toxicants not functioning on AChE. This method offered alternative choices for indicating the existence of enzyme inhibitors.
学科领域分析化学
部门归属中科院上海有机化学研究所; 上海市公安局刑事科学技术研究管理中心
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收录类别SCI
语种英语
WOS记录号WOS:000307786900021
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被引频次:6[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28657
专题分析化学研究室
通讯作者Zhang L(张立); Zhang YR(张玉荣)
推荐引用方式
GB/T 7714
Cai TT,Zhang L,Wang R,et al. Rapid Detection of Irreversible Acetylcholineasterase Inhibitor by Mass Spectrometry Assay[J]. Chin. J. Chem.,2012,30(8):1788-1792.
APA 蔡婷婷.,张立.,汪蓉.,梁晨.,赵武生.,...&郭寅龙.(2012).Rapid Detection of Irreversible Acetylcholineasterase Inhibitor by Mass Spectrometry Assay.Chin. J. Chem.,30(8),1788-1792.
MLA 蔡婷婷,et al."Rapid Detection of Irreversible Acetylcholineasterase Inhibitor by Mass Spectrometry Assay".Chin. J. Chem. 30.8(2012):1788-1792.
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