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学科主题: 分析化学
题名: Rapid Detection of Irreversible Acetylcholineasterase Inhibitor by Mass Spectrometry Assay
其他题名: 质谱法快速检测不可逆乙酰胆碱酯酶抑制剂
作者: Cai TT(蔡婷婷) ; Zhang L(张立) ; Wang R(汪蓉) ; Liang C(梁晨) ; Zhao WS(赵武生) ; Fu DF(傅得锋) ; Zhang YR(张玉荣) ; Guo YL(郭寅龙)
通讯作者: 张立 ; 张玉荣
刊名: Chin. J. Chem.
发表日期: 2012
卷: 30, 期:8, 页:1788-1792
收录类别: SCI
部门归属: 中科院上海有机化学研究所; 上海市公安局刑事科学技术研究管理中心
英文摘要: Here we developed a rapid method to detect acetylcholinesterase (AChE) activity by matrix-assisted laser desorption/ionization Fourier transform mass spectrometry (MALDI-FTMS) for screening irreversible AChE inhibitors. Due to its good salt-tolerance and low sample consumption, MALDI-FTMS could facilitate rapid detection, especially detection in real application. AChE activity was determined through calculating abundance of substrate and product in mass spectrometry. By this approach, we investigated the relation of organophosphorous (OP) concentrations and AChE inhibition. Shown in different inhibition curves from different OP pesticides, enzyme inhibitions still kept good correlation with concentration of OPs. Finally, this AChE-inhibited method was applied to screen whole bloods of four decedents and discuss their death reason. In contrast to healthy persons, three of decedents showed low AChE activity, and probably died for irreversible AChE inhibitors. Through the following detecting in GC-MS/MS, the possible death reason of these three decedents was confirmed, and another decedent actually died for sumicidin, a non-AChE inhibitor. It demonstrated that screening irreversible AChE inhibitors by detecting enzyme activity in MALDI-FTMS provided fast and accurate analysis results and excluded another toxicants not functioning on AChE. This method offered alternative choices for indicating the existence of enzyme inhibitors.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000307786900021
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28657
Appears in Collections:分析化学研究室_期刊论文

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Recommended Citation:
Cai TT,Zhang L,Wang R,et al. Rapid Detection of Irreversible Acetylcholineasterase Inhibitor by Mass Spectrometry Assay[J]. Chin. J. Chem.,2012,30(8):1788-1792.
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