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学科主题: 生命有机化学
题名: In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O
其他题名: 通过鉴定番红霉素O的结构体内研究SfmO2在番红霉素A生物合成中的功能
作者: Peng C(彭超) ; Tang YM(唐玉敏) ; Li L(李磊) ; Ding W(丁伟) ; Deng W(邓玮) ; Pu JY(蒲津越) ; Liu W(刘文) ; Tang GL(唐功利)
通讯作者: 唐功利
刊名: Sci. China-Chem.
发表日期: 2012
卷: 55, 期:1, 页:90-97
收录类别: SCI
部门归属: 中科院上海有机化学研究所
英文摘要: Saframycin A (SFM-A), a tetrahydroisoquinoline antibiotic isolated from Streptomyces lavendulae, shows potent anti-proliferation activities against a variety of tumor cell lines, and shares the core structure with ecteinascidin 743 (ET-743), the anticancer drug for soft-tissue sarcoma. Characterization of the SFM-A biosynthetic gene cluster revealed three nonribosomal peptide synthetase genes and a series of genes encoding oxygenases. To investigate the function of sfmO2 gene, encoding a FAD-dependent monooxygenase/hydroxylase, we constructed the gene replacement mutant (Delta sfmO2) strain S. lavendulae TL2007 and the corresponding gene complementation mutant strain S. lavendulae TL2008. A novel compound, SFM-O, was isolated from the Delta sfmO2 replacement mutant strain and its structure was characterized by comparison to the HRMS and NMR spectra of SFM-A. These findings indicated that SfmO2 is responsible for the oxidation of ring A in the biosynthetic pathway of SFM-A, and the new compound SFM-O could be considered as an advanced intermediate in the semisynthesis of ET-743.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000298968200012
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28551
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Peng C,Tang YM,Li L,et al. In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O[J]. Sci. China-Chem.,2012,55(1):90-97.
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