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In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O
其他题名通过鉴定番红霉素O的结构体内研究SfmO2在番红霉素A生物合成中的功能
Peng C(彭超); Tang YM(唐玉敏); Li L(李磊); Ding W(丁伟); Deng W(邓玮); Pu JY(蒲津越); Liu W(刘文); Tang GL(唐功利)
2012
发表期刊Sci. China-Chem.
ISSN1674-7291
卷号55期号:1页码:90-97
摘要Saframycin A (SFM-A), a tetrahydroisoquinoline antibiotic isolated from Streptomyces lavendulae, shows potent anti-proliferation activities against a variety of tumor cell lines, and shares the core structure with ecteinascidin 743 (ET-743), the anticancer drug for soft-tissue sarcoma. Characterization of the SFM-A biosynthetic gene cluster revealed three nonribosomal peptide synthetase genes and a series of genes encoding oxygenases. To investigate the function of sfmO2 gene, encoding a FAD-dependent monooxygenase/hydroxylase, we constructed the gene replacement mutant (Delta sfmO2) strain S. lavendulae TL2007 and the corresponding gene complementation mutant strain S. lavendulae TL2008. A novel compound, SFM-O, was isolated from the Delta sfmO2 replacement mutant strain and its structure was characterized by comparison to the HRMS and NMR spectra of SFM-A. These findings indicated that SfmO2 is responsible for the oxidation of ring A in the biosynthetic pathway of SFM-A, and the new compound SFM-O could be considered as an advanced intermediate in the semisynthesis of ET-743.
学科领域生命有机化学
部门归属中科院上海有机化学研究所
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收录类别SCI
语种英语
WOS记录号WOS:000298968200012
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被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28551
专题生命有机化学国家重点实验室
通讯作者Tang GL(唐功利)
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Peng C,Tang YM,Li L,et al. In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O[J]. Sci. China-Chem.,2012,55(1):90-97.
APA 彭超.,唐玉敏.,李磊.,丁伟.,邓玮.,...&唐功利.(2012).In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O.Sci. China-Chem.,55(1),90-97.
MLA 彭超,et al."In vivo investigation of the role of SfmO2 in saframycin A biosynthesis by structural characterization of the analogue saframycin O".Sci. China-Chem. 55.1(2012):90-97.
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