中国科学院上海有机化学研究所机构知识库
Advanced  
SIOC OpenIR  > 生命有机化学国家重点实验室  > 期刊论文
学科主题: 生命有机化学
题名: Analysis of YM-216391 Biosynthetic Gene Cluster and Improvement of the Cyclopeptide Production in a Heterologous Host
其他题名: 分析YM-216391生物合成基因簇并在异源宿主中提高产量
作者: Jian XH(蹇晓红) ; Pan HX(潘海学) ; Ning TT(宁婷婷) ; Shi YY(侍媛媛) ; Chen YS(陈永升) ; Li Y(李燕) ; Ceng XW(曾晓伟) ; Xu J(徐健) ; Tang GL(唐功利)
通讯作者: 唐功利
刊名: ACS Chem. Biol.
发表日期: 2012
卷: 7, 期:4, 页:646-651
收录类别: SCI
部门归属: 中科院上海有机化学研究所; 中科院青岛能源所
英文摘要: YM-216391, an autitumor natural product, represents a new class of cyclic peptides containing a polyoxazolethiazole moiety, Herein we describe its gene cluster encoding the biosynthetic paradigm featuring a ribosomally synthesizing procursor peptide followed by a series of novel posttranslational modifications which include (1) cleavage of both N-terminal leader peptide and C-terminal extension peptide and cyclization in a head-to-tail fashion, (ii) conversion of an L-Ile to D-allo-Ile, and (iii) beta-hydroxylation of Phe by a P450 monooxygenase followed by further heterocyclization and oxidation to form a phenyloxazole moiety. The cluster was heterologously expressed in Streptomyces lividans to bypass difficult genetic manipulation. Deletion of the ymR3 gene, encoding a putative transcriptional regulator, increased the YM-216391 yield about 20-fold higher than the original yields for the heterologous expression of wild-type cluster, which set the stage for further combinatorial biosynthesis.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000302977300004
Citation statistics:
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28535
Appears in Collections:生命有机化学国家重点实验室_期刊论文

Files in This Item: Download All
File Name/ File Size Content Type Version Access License
2012138.pdf(1542KB)----开放获取--View Download

Recommended Citation:
Jian XH,Pan HX,Ning TT,et al. Analysis of YM-216391 Biosynthetic Gene Cluster and Improvement of the Cyclopeptide Production in a Heterologous Host[J]. ACS Chem. Biol.,2012,7(4):646-651.
Service
Recommend this item
Sava as my favorate item
Show this item's statistics
Export Endnote File
Google Scholar
Similar articles in Google Scholar
[蹇晓红]'s Articles
[潘海学]'s Articles
[宁婷婷]'s Articles
CSDL cross search
Similar articles in CSDL Cross Search
[蹇晓红]‘s Articles
[潘海学]‘s Articles
[宁婷婷]‘s Articles
Related Copyright Policies
Null
Social Bookmarking
Add to CiteULike Add to Connotea Add to Del.icio.us Add to Digg Add to Reddit
文件名: 2012138.pdf
格式: Adobe PDF
此文件暂不支持浏览
所有评论 (0)
暂无评论
 
评注功能仅针对注册用户开放,请您登录
您对该条目有什么异议,请填写以下表单,管理员会尽快联系您。
内 容:
Email:  *
单位:
验证码:   刷新
您在IR的使用过程中有什么好的想法或者建议可以反馈给我们。
标 题:
 *
内 容:
Email:  *
验证码:   刷新

Items in IR are protected by copyright, with all rights reserved, unless otherwise indicated.

 

 

Valid XHTML 1.0!
Powered by CSpace