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Analysis of YM-216391 Biosynthetic Gene Cluster and Improvement of the Cyclopeptide Production in a Heterologous Host
其他题名分析YM-216391生物合成基因簇并在异源宿主中提高产量
Jian XH(蹇晓红); Pan HX(潘海学); Ning TT(宁婷婷); Shi YY(侍媛媛); Chen YS(陈永升); Li Y(李燕); Ceng XW(曾晓伟); Xu J(徐健); Tang GL(唐功利)
2012
发表期刊ACS Chem. Biol.
ISSN1554-8929
卷号7期号:4页码:646-651
摘要YM-216391, an autitumor natural product, represents a new class of cyclic peptides containing a polyoxazolethiazole moiety, Herein we describe its gene cluster encoding the biosynthetic paradigm featuring a ribosomally synthesizing procursor peptide followed by a series of novel posttranslational modifications which include (1) cleavage of both N-terminal leader peptide and C-terminal extension peptide and cyclization in a head-to-tail fashion, (ii) conversion of an L-Ile to D-allo-Ile, and (iii) beta-hydroxylation of Phe by a P450 monooxygenase followed by further heterocyclization and oxidation to form a phenyloxazole moiety. The cluster was heterologously expressed in Streptomyces lividans to bypass difficult genetic manipulation. Deletion of the ymR3 gene, encoding a putative transcriptional regulator, increased the YM-216391 yield about 20-fold higher than the original yields for the heterologous expression of wild-type cluster, which set the stage for further combinatorial biosynthesis.
学科领域生命有机化学
部门归属中科院上海有机化学研究所; 中科院青岛能源所
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收录类别SCI
语种英语
WOS记录号WOS:000302977300004
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被引频次:14[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28535
专题生命有机化学国家重点实验室
通讯作者Tang GL(唐功利)
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GB/T 7714
Jian XH,Pan HX,Ning TT,et al. Analysis of YM-216391 Biosynthetic Gene Cluster and Improvement of the Cyclopeptide Production in a Heterologous Host[J]. ACS Chem. Biol.,2012,7(4):646-651.
APA 蹇晓红.,潘海学.,宁婷婷.,侍媛媛.,陈永升.,...&唐功利.(2012).Analysis of YM-216391 Biosynthetic Gene Cluster and Improvement of the Cyclopeptide Production in a Heterologous Host.ACS Chem. Biol.,7(4),646-651.
MLA 蹇晓红,et al."Analysis of YM-216391 Biosynthetic Gene Cluster and Improvement of the Cyclopeptide Production in a Heterologous Host".ACS Chem. Biol. 7.4(2012):646-651.
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