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Arabidopsis Acetyl-Amido Synthetase GH3.5 Involvement in Camalexin Biosynthesis through Conjugation of Indole-3-Carboxylic Acid and Cysteine and Upregulation of Camalexin Biosynthesis Genes
其他题名拟南芥乙酰基-酰氨基合成酶GH3.5通过吲哚-3-羧酸和半胱氨酸耦合及camalexin生物合成基因上调参与camalexin的生物合成
WANG MUYANG; Liu XT(刘雪婷); CHEN YING; XU XIAOJING; Yu B(俞飚); ZHANG SHUQUN; LI QUN; HE ZUHUA
2012
发表期刊J. Integr. Plant Biol.
ISSN1672-9072
卷号54期号:7页码:471-485
摘要Camalexin (3-thiazol-2'-yl-indole) is the major phytoalexin found in Arabidopsis thaliana. Several key intermediates and corresponding enzymes have been identified in camalexin biosynthesis through mutant screening and biochemical experiments. Camalexin is formed when indole-3-acetonitrile (IAN) is catalyzed by the cytochrome P450 monooxygenase CYP71A13. Here, we demonstrate that the Arabidopsis GH3.5 protein, a multifunctional acetyl-amido synthetase, is involved in camalexin biosynthesis via conjugating indole-3-carboxylic acid (ICA) and cysteine (Cys) and regulating camalexin biosynthesis genes. Camalexin levels were increased in the activation-tagged mutant gh3.51D in both Col-0 and cyp71A132 mutant backgrounds after pathogen infection. The recombinant GH3.5 protein catalyzed the conjugation of ICA and Cys to form a possible intermediate indole-3-acyl-cysteinate (ICA(Cys)) in vitro. In support of the in vitro reaction, feeding with ICA and Cys increased camalexin levels in Col-0 and gh3.51D. Dihydrocamalexic acid (DHCA), the precursor of camalexin and the substrate for PAD3, was accumulated in gh3.51D/pad31, suggesting that ICA(Cys) could be an additional precursor of DHCA for camalexin biosynthesis. Furthermore, expression of the major camalexin biosynthesis genes CYP79B2, CYP71A12, CYP71A13 and PAD3 was strongly induced in gh3.51D. Our study suggests that GH3.5 is involved in camalexin biosynthesis through direct catalyzation of the formation of ICA(Cys), and upregulation of the major biosynthetic pathway genes.
学科领域生命有机化学
部门归属中科院植生所; 中科院上海有机化学研究所; 上海生物芯片工程中心; 密苏里大学
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收录类别SCI
语种英语
WOS记录号WOS:000306570900004
引用统计
被引频次:16[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28495
专题生命有机化学国家重点实验室
通讯作者LI QUN; HE ZUHUA
推荐引用方式
GB/T 7714
WANG MUYANG,Liu XT,CHEN YING,et al. Arabidopsis Acetyl-Amido Synthetase GH3.5 Involvement in Camalexin Biosynthesis through Conjugation of Indole-3-Carboxylic Acid and Cysteine and Upregulation of Camalexin Biosynthesis Genes[J]. J. Integr. Plant Biol.,2012,54(7):471-485.
APA WANG MUYANG.,刘雪婷.,CHEN YING.,XU XIAOJING.,俞飚.,...&HE ZUHUA.(2012).Arabidopsis Acetyl-Amido Synthetase GH3.5 Involvement in Camalexin Biosynthesis through Conjugation of Indole-3-Carboxylic Acid and Cysteine and Upregulation of Camalexin Biosynthesis Genes.J. Integr. Plant Biol.,54(7),471-485.
MLA WANG MUYANG,et al."Arabidopsis Acetyl-Amido Synthetase GH3.5 Involvement in Camalexin Biosynthesis through Conjugation of Indole-3-Carboxylic Acid and Cysteine and Upregulation of Camalexin Biosynthesis Genes".J. Integr. Plant Biol. 54.7(2012):471-485.
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