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学科主题: 生命有机化学
题名: Arabidopsis Acetyl-Amido Synthetase GH3.5 Involvement in Camalexin Biosynthesis through Conjugation of Indole-3-Carboxylic Acid and Cysteine and Upregulation of Camalexin Biosynthesis Genes
其他题名: 拟南芥乙酰基-酰氨基合成酶GH3.5通过吲哚-3-羧酸和半胱氨酸耦合及camalexin生物合成基因上调参与camalexin的生物合成
作者: WANG MUYANG ; Liu XT(刘雪婷) ; CHEN YING ; XU XIAOJING ; Yu B(俞飚) ; ZHANG SHUQUN ; LI QUN ; HE ZUHUA
通讯作者: LI QUN ; HE ZUHUA
刊名: J. Integr. Plant Biol.
发表日期: 2012
卷: 54, 期:7, 页:471-485
收录类别: SCI
部门归属: 中科院植生所; 中科院上海有机化学研究所; 上海生物芯片工程中心; 密苏里大学
英文摘要: Camalexin (3-thiazol-2'-yl-indole) is the major phytoalexin found in Arabidopsis thaliana. Several key intermediates and corresponding enzymes have been identified in camalexin biosynthesis through mutant screening and biochemical experiments. Camalexin is formed when indole-3-acetonitrile (IAN) is catalyzed by the cytochrome P450 monooxygenase CYP71A13. Here, we demonstrate that the Arabidopsis GH3.5 protein, a multifunctional acetyl-amido synthetase, is involved in camalexin biosynthesis via conjugating indole-3-carboxylic acid (ICA) and cysteine (Cys) and regulating camalexin biosynthesis genes. Camalexin levels were increased in the activation-tagged mutant gh3.51D in both Col-0 and cyp71A132 mutant backgrounds after pathogen infection. The recombinant GH3.5 protein catalyzed the conjugation of ICA and Cys to form a possible intermediate indole-3-acyl-cysteinate (ICA(Cys)) in vitro. In support of the in vitro reaction, feeding with ICA and Cys increased camalexin levels in Col-0 and gh3.51D. Dihydrocamalexic acid (DHCA), the precursor of camalexin and the substrate for PAD3, was accumulated in gh3.51D/pad31, suggesting that ICA(Cys) could be an additional precursor of DHCA for camalexin biosynthesis. Furthermore, expression of the major camalexin biosynthesis genes CYP79B2, CYP71A12, CYP71A13 and PAD3 was strongly induced in gh3.51D. Our study suggests that GH3.5 is involved in camalexin biosynthesis through direct catalyzation of the formation of ICA(Cys), and upregulation of the major biosynthetic pathway genes.
语种: 英语
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WOS记录号: WOS:000306570900004
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28495
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
WANG MUYANG,Liu XT,CHEN YING,et al. Arabidopsis Acetyl-Amido Synthetase GH3.5 Involvement in Camalexin Biosynthesis through Conjugation of Indole-3-Carboxylic Acid and Cysteine and Upregulation of Camalexin Biosynthesis Genes[J]. J. Integr. Plant Biol.,2012,54(7):471-485.
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