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学科主题: 生命有机化学
题名: Nosiheptide Biosynthesis Featuring a Unique Indole Side Ring Formation on the Characteristic Thiopeptide Framework
其他题名: Nosiheptide Biosynthesis Featuring a Unique Indole Side Ring Formation on the Characteristic Thiopeptide Framework
作者: Yu Y(虞沂) ; DUAN LIAN ; Zhang Q(张琪) ; LIAO RIJING ; DING YING ; PAN HAIXUE ; WENDTPIENKOWSKI EVELYN ; Tang GL(唐功利) ; SHEN BEN ; Liu W(刘文)
通讯作者: 刘文
刊名: ACS Chem. Biol.
发表日期: 2009
卷: 4, 期:10, 页:855-864
收录类别: SCI
部门归属: 上海有机所; 上海交通大学; 美国威斯康星大学
英文摘要: Nosiheptide (NOS), belonging to thee series of thiopeptide antibiotics that exhibit potent activity against various bacterial pathogens, bears a unique indole side ring system and regiospecific hydroxyl groups on the characteristic macrocyclic core. Here, cloning, sequencing, and characterization of the nos gene cluster from Streptomyces actuosus ATCC 25421 as a model for this series of thiopeptides has unveiled new insights Into their biosynthesis. Bioinformatics-based sequence analysis and In vivo Investigation Into the gene functions show that NOS biosynthesis shares a common strategy with recently characterized b or c series thiopeptides for forming the characteristic macrocyclic core, which features a ribosomally synthesized precursor peptide with conserved posttranslational modifications. However, it apparently proceeds via a different route for tailoring the thiopeptide framework, allowing the final product to exhibit the distinct structural characteristics of e series thiopeptides, such as the indole side ring system. Chemical complementation supports the notion that the S-adenosylmethionine-dependent protein NosL may play a central role in converting tryptophan to the key 3-methylindole moiety by an unusual carbon side chain rearrangement, most likely via a radical-initiated mechanism. Characterization of the indole side ring-opened analogue of NOS from the nosN mutant strain Is consistent with the proposed methyltransferase activity of Its encoded protein, shedding light into the timing of the individual steps for Indole side ring biosynthesis. These results also suggest the feasibility of engineering novel thiopeptides for drug discovery by manipulating the NOS biosynthetic machinery.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000272562000006
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28455
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Yu Y,DUAN LIAN,Zhang Q,et al. Nosiheptide Biosynthesis Featuring a Unique Indole Side Ring Formation on the Characteristic Thiopeptide Framework[J]. ACS Chem. Biol.,2009,4(10):855-864.
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