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学科主题: 生命有机化学
题名: Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway
其他题名: Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway
作者: FU CHENGYU ; Tang MC(唐满成) ; Peng C(彭超) ; Li L(李磊) ; HE YANLING ; Liu W(刘文) ; Tang GL(唐功利)
通讯作者: 唐功利
刊名: J. Microbiol. Biotechnol.
发表日期: 2009
卷: 19, 期:5, 页:439-446
收录类别: SCI
部门归属: 上海有机所; 西安交通大学
英文摘要: The biosynthesis study of antibiotics saframycin (SFM) in Streptomyces lavendulae and safracin (SAC) in Pseudomonas fluorescens demonstrated that 3-hydroxy-5-methyl-O-methyltyrosine (3h5mOmTyr), a nonproteinogenic amino acid, is the precursor of the tetra hydroisoquinoline molecular core. In the biosynthetic gene cluster of SAC/SFM, sacD/sfmD encodes a protein with high homology to each other but no sequence similarity to other known enzymes; sacF/sfmM2 and sacG/sfmM3 encode methyltransferases for C-methylation and O-methylation; and sacE/sfmF encodes a small protein with significant sequence similarity to the MbtH-like proteins, which are frequently found in the biosynthetic pathways of nonribosomal peptide antibiotics and siderophores. To address their function, the biosynthetic cassette of 3h5mOmTyr was heterologously expressed in S. coelicolor and P. putida, and an in-frame deletion and complementation in trans were carried out. The results revealed that (i) SfmD catalyzes the hydroxylation of aromatic rings; (ii) sacD/sacF/sacG in the SAC gene cluster and sfmD/sfmM2/sfmM3 in the SFM cluster are sufficient for the biosynthesis of 3h5mOmTyr; and (iii) the mbtH-like gene is not required for the biosynthesis of the 3h5mOmTyr precursor.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000266447800002
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28449
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
FU CHENGYU,Tang MC,Peng C,et al. Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway[J]. J. Microbiol. Biotechnol.,2009,19(5):439-446.
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