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Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway
其他题名Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway
FU CHENGYU; Tang MC(唐满成); Peng C(彭超); Li L(李磊); HE YANLING; Liu W(刘文); Tang GL(唐功利)
2009
发表期刊J. Microbiol. Biotechnol.
ISSN1017-7825
卷号19期号:5页码:439-446
摘要The biosynthesis study of antibiotics saframycin (SFM) in Streptomyces lavendulae and safracin (SAC) in Pseudomonas fluorescens demonstrated that 3-hydroxy-5-methyl-O-methyltyrosine (3h5mOmTyr), a nonproteinogenic amino acid, is the precursor of the tetra hydroisoquinoline molecular core. In the biosynthetic gene cluster of SAC/SFM, sacD/sfmD encodes a protein with high homology to each other but no sequence similarity to other known enzymes; sacF/sfmM2 and sacG/sfmM3 encode methyltransferases for C-methylation and O-methylation; and sacE/sfmF encodes a small protein with significant sequence similarity to the MbtH-like proteins, which are frequently found in the biosynthetic pathways of nonribosomal peptide antibiotics and siderophores. To address their function, the biosynthetic cassette of 3h5mOmTyr was heterologously expressed in S. coelicolor and P. putida, and an in-frame deletion and complementation in trans were carried out. The results revealed that (i) SfmD catalyzes the hydroxylation of aromatic rings; (ii) sacD/sacF/sacG in the SAC gene cluster and sfmD/sfmM2/sfmM3 in the SFM cluster are sufficient for the biosynthesis of 3h5mOmTyr; and (iii) the mbtH-like gene is not required for the biosynthesis of the 3h5mOmTyr precursor.
学科领域生命有机化学
部门归属上海有机所; 西安交通大学
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收录类别SCI
语种英语
WOS记录号WOS:000266447800002
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文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28449
专题生命有机化学国家重点实验室
通讯作者Tang GL(唐功利)
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GB/T 7714
FU CHENGYU,Tang MC,Peng C,et al. Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway[J]. J. Microbiol. Biotechnol.,2009,19(5):439-446.
APA FU CHENGYU.,唐满成.,彭超.,李磊.,HE YANLING.,...&唐功利.(2009).Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway.J. Microbiol. Biotechnol.,19(5),439-446.
MLA FU CHENGYU,et al."Biosynthesis of 3-Hydroxy-5-Methyl-O-Methyltyrosine in the Saframycin/Safracin Biosynthetic Pathway".J. Microbiol. Biotechnol. 19.5(2009):439-446.
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