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Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors
其他题名树枝状聚合物包裹的纳米金作为非病毒基因输送载体的研究
Dan YB(单岳滨); Luo T(罗挺); Peng C(彭琛); Sheng RL(盛瑞隆); Cao AM(曹阿民); Cao XY(曹雪燕); Shen MW(沈明武); Guo R(郭睿); Shi XY(史向阳)
2012
发表期刊Biomaterials
ISSN0142-9612
卷号33期号:10页码:3025-3035
摘要Development of highly efficient nonviral gene delivery vectors still remains a great challenge. In this study, we report a new gene delivery vector based on dendrimer-entrapped gold nanoparticles (Au DENPs) with significantly higher gene transfection efficiency than that of dendrimers without AuNPs entrapped. Amine-terminated generation 5 poly(amidoamine) (PAMAM) dendrimers (G5.NH2) were utilized as templates to synthesize AuNPs with different Au atom/dendrimer molar ratios (25:1, 50:1, 75:1, and 100:1, respectively). The formed Au DENPs were used to complex two different pDNAs encoding luciferase (Luc) and enhanced green fluorescent protein (EGFP), respectively for gene transfection studies. The Au DENPs/pDNA polyplexes with different N/P ratios and compositions of Au DENPs were characterized by gel retardation assay, light scattering, zeta potential measurements, and atomic force microscopic imaging. We show that the Au DENPs can effectively compact the pDNA, allowing for highly efficient gene transfection into the selected cell lines as demonstrated by both Luc assay and fluorescence microscopic imaging of the ECFP expression. The transfection efficiency of Au DENPs with Au atom/dendrimer molar ratio of 25:1 was at least 100 times higher than that of G5.NH2 dendrimers without AuNPs entrapped at the N/P ratio of 2.5:1. The higher gene transfection efficiency of Au DENPs is primarily due to the fact that the entrapment of AuNPs helps preserve the 3-dimensional spherical morphology of dendrimers, allowing for more efficient interaction between dendrimers and DNA. With the less cytotoxicity than that of G5.NH2 dendrimers demonstrated by thiazoyl blue tetrazolium bromide assay and higher gene transfection efficiency, it is expected that Au DENPs may be used as a new gene delivery vector for highly efficient transfection of different genes for various biomedical applications. (C) 2011 Elsevier Ltd. All rights reserved.
学科领域高分子化学 ; 生命有机化学
部门归属东华大学; 中科院上海有机化学研究所; Univ Madeira
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000300920900020
引用统计
被引频次:132[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/28405
专题高分子材料研究室
通讯作者Cao AM(曹阿民); Shi XY(史向阳)
推荐引用方式
GB/T 7714
Dan YB,Luo T,Peng C,et al. Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors[J]. Biomaterials,2012,33(10):3025-3035.
APA 单岳滨.,罗挺.,彭琛.,盛瑞隆.,曹阿民.,...&史向阳.(2012).Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors.Biomaterials,33(10),3025-3035.
MLA 单岳滨,et al."Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors".Biomaterials 33.10(2012):3025-3035.
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