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学科主题: 高分子化学 ; 生命有机化学
题名: Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors
其他题名: 树枝状聚合物包裹的纳米金作为非病毒基因输送载体的研究
作者: Dan YB(单岳滨) ; Luo T(罗挺) ; Peng C(彭琛) ; Sheng RL(盛瑞隆) ; Cao AM(曹阿民) ; Cao XY(曹雪燕) ; Shen MW(沈明武) ; Guo R(郭睿) ; Shi XY(史向阳)
通讯作者: 曹阿民 ; 史向阳
刊名: Biomaterials
发表日期: 2012
卷: 33, 期:10, 页:3025-3035
收录类别: SCI
部门归属: 东华大学; 中科院上海有机化学研究所; Univ Madeira
英文摘要: Development of highly efficient nonviral gene delivery vectors still remains a great challenge. In this study, we report a new gene delivery vector based on dendrimer-entrapped gold nanoparticles (Au DENPs) with significantly higher gene transfection efficiency than that of dendrimers without AuNPs entrapped. Amine-terminated generation 5 poly(amidoamine) (PAMAM) dendrimers (G5.NH2) were utilized as templates to synthesize AuNPs with different Au atom/dendrimer molar ratios (25:1, 50:1, 75:1, and 100:1, respectively). The formed Au DENPs were used to complex two different pDNAs encoding luciferase (Luc) and enhanced green fluorescent protein (EGFP), respectively for gene transfection studies. The Au DENPs/pDNA polyplexes with different N/P ratios and compositions of Au DENPs were characterized by gel retardation assay, light scattering, zeta potential measurements, and atomic force microscopic imaging. We show that the Au DENPs can effectively compact the pDNA, allowing for highly efficient gene transfection into the selected cell lines as demonstrated by both Luc assay and fluorescence microscopic imaging of the ECFP expression. The transfection efficiency of Au DENPs with Au atom/dendrimer molar ratio of 25:1 was at least 100 times higher than that of G5.NH2 dendrimers without AuNPs entrapped at the N/P ratio of 2.5:1. The higher gene transfection efficiency of Au DENPs is primarily due to the fact that the entrapment of AuNPs helps preserve the 3-dimensional spherical morphology of dendrimers, allowing for more efficient interaction between dendrimers and DNA. With the less cytotoxicity than that of G5.NH2 dendrimers demonstrated by thiazoyl blue tetrazolium bromide assay and higher gene transfection efficiency, it is expected that Au DENPs may be used as a new gene delivery vector for highly efficient transfection of different genes for various biomedical applications. (C) 2011 Elsevier Ltd. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000300920900020
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/28405
Appears in Collections:高分子材料研究室_期刊论文

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Recommended Citation:
Dan YB,Luo T,Peng C,et al. Gene delivery using dendrimer-entrapped gold nanoparticles as nonviral vectors[J]. Biomaterials,2012,33(10):3025-3035.
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