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Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation COMMUNICATIONS
其他题名参与DNA磷硫修饰的DndE蛋白(来源: 沙门氏菌血清型Cerror 87)表达、结晶及初步X衍射分析
CHEN FUKUN; LIN KUI; ZHANG ZHENYI; CHEN LEYI; SHI XIAOSHAN; Cao CY(曹春阳); WANG ZHIJUN; LIANG JINGDAN; DENG ZIXIN; WU GENG
2011
发表期刊Acta Crystallogr. F-Struct. Biol. Cryst. Commun.
ISSN1744-3091
卷号67页码:1440-1442
摘要The phenomenon of DNA phosphorothioation (DNA sulfur modification) is widespread among prokaryotes and may serve as a mechanism to restrict gene transfer among bacteria. DndE is one of five essential proteins that are required for the DNA phosphorothioation process. However, its exact biochemical role in sulfur modification of DNA remains unclear. In this study, the DndE protein homologue from Salmonella enterica serovar Cerro 87 was overexpressed, purified and crystallized. The crystals of the DndE protein diffracted to 2.7 angstrom resolution and belonged to space group P3(1)21. These results will facilitate detailed structural analysis of DndE and further elucidation of its biochemical function.
学科领域生命有机化学
部门归属上海交通大学; 中国科学院上海有机化学研究所
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收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/27827
专题上海有机化学研究所
通讯作者LIN KUI; WU GENG
推荐引用方式
GB/T 7714
CHEN FUKUN,LIN KUI,ZHANG ZHENYI,et al. Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation COMMUNICATIONS[J]. Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,2011,67:1440-1442.
APA CHEN FUKUN.,LIN KUI.,ZHANG ZHENYI.,CHEN LEYI.,SHI XIAOSHAN.,...&WU GENG.(2011).Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation COMMUNICATIONS.Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,67,1440-1442.
MLA CHEN FUKUN,et al."Purification, crystallization and preliminary X-ray analysis of the DndE protein from Salmonella enterica serovar Cerro 87, which is involved in DNA phosphorothioation COMMUNICATIONS".Acta Crystallogr. F-Struct. Biol. Cryst. Commun. 67(2011):1440-1442.
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