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Purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda COMMUNICATIONS
Alternative Title条斑紫菜中藻红蛋白和藻蓝蛋白的纯化、结晶及初步X射线晶体学分析
Cai C(蔡春); Wu L(吴联); Li CX(李春霞); He PM(何培民); Li J(李洁); Zhou JH(周佳海)
2011
Source PublicationActa Crystallogr. F-Struct. Biol. Cryst. Commun.
ISSN1744-3091
Volume67Pages:579-583
AbstractPorphyra yezoensis is one of the most important and widely cultured seaweeds in China. Phycobiliproteins exhibit excellent spectroscopic properties and play versatile roles in the biomedical, food, cosmetics and chemical synthetic dye industries. Here, the purification and crystallization of phycoerythrin and phycocyanin, two phycobiliproteins extracted from P. yezoensis, are described. Using a novel protocol including co-precipitation with ammonium sulfate and hydroxyapatite column chromatography, both phycobiliproteins were produced on a large scale with improved quality and yield compared with those previously reported. Native PAGE analysis indicated that phycoerythrin and phycocyanin exist as (alpha beta)(3) heterohexamers in solution. The crystals of phycoerythrin diffracted to 2.07 angstrom resolution and belonged to space group R3. The unit-cell parameters referred to hexagonal axes are a = b = 187.7, c = 59.7 angstrom, with nine (alpha beta)(2) heterotetramers per unit cell. The crystals of phycocyanin diffracted to 2.70 angstrom resolution in space group P2(1). Matthews coefficient analysis shows that 10-19 (alpha beta) heterodimers of phycocyanin in the asymmetric unit would be reasonable. A self-rotation function calculation clarified this ambiguity and indicated that 12 (alpha beta) heterodimers of phycocyanin are assembled in the asymmetric unit.
Subject Area生命有机化学
Department中科院上海有机化学研究所; 上海海洋大学
URL查看原文
Indexed BySCI
Language英语
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/27819
Collection上海有机化学研究所
Corresponding AuthorLi J(李洁)
Recommended Citation
GB/T 7714
Cai C,Wu L,Li CX,et al. Purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda COMMUNICATIONS[J]. Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,2011,67:579-583.
APA 蔡春,吴联,李春霞,何培民,李洁,&周佳海.(2011).Purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda COMMUNICATIONS.Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,67,579-583.
MLA 蔡春,et al."Purification, crystallization and preliminary X-ray analysis of phycocyanin and phycoerythrin from Porphyra yezoensis Ueda COMMUNICATIONS".Acta Crystallogr. F-Struct. Biol. Cryst. Commun. 67(2011):579-583.
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