SIOC OpenIR  > 上海有机化学研究所
Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios
其他题名Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios
ZHANG QI; WU J; QIAN JIANGCHAO; CHU J; ZHUANG Y; ZHANG S; Liu W(刘文)
2011
发表期刊Lett. Appl. Microbiol.
ISSN0266-8254
卷号52期号:2页码:129-137
摘要Aims: To overproduce erythromycin C, B or D and evaluate the effect of disruption of tailoring genes eryK and eryG in an industrial erythromycin producer. Methods and Results: The tailoring genes eryG and eryK were inactivated individually or simultaneously by targeted gene disruption in an industrial strain Saccharopolyspora erythraea HL3168 E3, resulting in the overproduction of erythromycin C (2 center dot 48 g l-1), B (1 center dot 70 g l-1) or D (2 center dot 15 g l-1) in the mutant strain QL-G, QL-K or QL-KG, respectively. Analysis of the erythromycin congeners throughout the fermentation indicated that, at the end of fermentation, comparatively large amount of erythromycin D (0 center dot 67 g l-1) was accumulated in QL-G, whereas only small amount of erythromycin D (0 center dot 10 g l-1) was produced in QL-K. Conclusions: Inactivation of tailoring genes eryG and eryK in the high producer did not affect the biosynthesis of erythromycin. However, erythromycin D could be more efficiently methylated by EryG than be hydroxylated by EryK. Significance and Impact of the Study: Development of the mutant strains provides a method for the economical large-scale production of potent lead compounds. The information about the accumulation and conversion of erythromycins in the industrial strains may contribute to further improving erythromycin production.
学科领域生命有机化学
部门归属华东理工大学; 中科院上海有机化学研究所
URL查看原文
收录类别SCI
语种英语
WOS记录号WOS:000286003400007
引用统计
被引频次:3[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/27815
专题上海有机化学研究所
通讯作者QIAN JIANGCHAO; Liu W(刘文)
推荐引用方式
GB/T 7714
ZHANG QI,WU J,QIAN JIANGCHAO,et al. Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios[J]. Lett. Appl. Microbiol.,2011,52(2):129-137.
APA ZHANG QI.,WU J.,QIAN JIANGCHAO.,CHU J.,ZHUANG Y.,...&刘文.(2011).Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios.Lett. Appl. Microbiol.,52(2),129-137.
MLA ZHANG QI,et al."Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios".Lett. Appl. Microbiol. 52.2(2011):129-137.
条目包含的文件
文件名称/大小 文献类型 版本类型 开放类型 使用许可
2011130.pdf(618KB) 开放获取--请求全文
个性服务
推荐该条目
保存到收藏夹
查看访问统计
导出为Endnote文件
谷歌学术
谷歌学术中相似的文章
[ZHANG QI]的文章
[WU J]的文章
[QIAN JIANGCHAO]的文章
百度学术
百度学术中相似的文章
[ZHANG QI]的文章
[WU J]的文章
[QIAN JIANGCHAO]的文章
必应学术
必应学术中相似的文章
[ZHANG QI]的文章
[WU J]的文章
[QIAN JIANGCHAO]的文章
相关权益政策
暂无数据
收藏/分享
所有评论 (0)
暂无评论
 

除非特别说明,本系统中所有内容都受版权保护,并保留所有权利。