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学科主题: 生命有机化学
题名: Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios
其他题名: Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios
作者: ZHANG QI ; WU J ; QIAN JIANGCHAO ; CHU J ; ZHUANG Y ; ZHANG S ; Liu W(刘文)
通讯作者: QIAN JIANGCHAO ; 刘文
刊名: Lett. Appl. Microbiol.
发表日期: 2011
卷: 52, 期:2, 页:129-137
收录类别: SCI
部门归属: 华东理工大学; 中科院上海有机化学研究所
英文摘要: Aims: To overproduce erythromycin C, B or D and evaluate the effect of disruption of tailoring genes eryK and eryG in an industrial erythromycin producer. Methods and Results: The tailoring genes eryG and eryK were inactivated individually or simultaneously by targeted gene disruption in an industrial strain Saccharopolyspora erythraea HL3168 E3, resulting in the overproduction of erythromycin C (2 center dot 48 g l-1), B (1 center dot 70 g l-1) or D (2 center dot 15 g l-1) in the mutant strain QL-G, QL-K or QL-KG, respectively. Analysis of the erythromycin congeners throughout the fermentation indicated that, at the end of fermentation, comparatively large amount of erythromycin D (0 center dot 67 g l-1) was accumulated in QL-G, whereas only small amount of erythromycin D (0 center dot 10 g l-1) was produced in QL-K. Conclusions: Inactivation of tailoring genes eryG and eryK in the high producer did not affect the biosynthesis of erythromycin. However, erythromycin D could be more efficiently methylated by EryG than be hydroxylated by EryK. Significance and Impact of the Study: Development of the mutant strains provides a method for the economical large-scale production of potent lead compounds. The information about the accumulation and conversion of erythromycins in the industrial strains may contribute to further improving erythromycin production.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000286003400007
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/27815
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
ZHANG QI,WU J,QIAN JIANGCHAO,et al. Knocking out of tailoring genes eryK and eryG in an industrial erythromycin-producing strain of Saccharopolyspora erythraea leading to overproduction of erythromycin B, C and D at different conversion ratios[J]. Lett. Appl. Microbiol.,2011,52(2):129-137.
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