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学科主题: 生命有机化学
题名: Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22
其他题名: 发现和分析在Streptomyces hygroscopicus 10-22中负责硫肽类抗生素环噻唑霉素形成的生物合成基因簇
作者: Wang J(王绛) ; Yu Y(虞沂) ; Tang KX(唐克轩) ; Liu W(刘文) ; He XY(贺新义) ; Huang X(黄曦) ; Deng ZX(邓子新)
通讯作者: 贺新义 ; 黄曦
刊名: Appl. Environ. Microbiol.
发表日期: 2010
卷: 76, 期:7, 页:2335-2344
收录类别: SCI
部门归属: 上海交通大学; 中国科学院上海有机化学研究所
英文摘要: Thiopeptide antibiotics are an important class of natural products resulting from posttranslational modifications of ribosomally synthesized peptides. Cyclothiazomycin is a typical thiopeptide antibiotic that has a unique bridged macrocyclic structure derived from an 18-amino-acid structural peptide. Here we reported cloning, sequencing, and heterologous expression of the cyclothiazomycin biosynthetic gene cluster from Streptomyces hygroscopicus 10-22. Remarkably, successful heterologous expression of a 22.7-kb gene cluster in Streptomyces lividans 1326 suggested that there is a minimum set of 15 open reading frames that includes all of the functional genes required for cyclothiazomycin production. Six genes of these genes, cltBCDEFG flanking the structural gene cltA, were predicted to encode the enzymes required for the main framework of cyclothiazomycin, and two enzymes encoded by a putative operon, cltMN, were hypothesized to participate in the tailoring step to generate the tertiary thioether, leading to the final cyclization of the bridged macrocyclic structure. This rigorous bioinformatics analysis based on heterologous expression of cyclothiazomycin resulted in an ideal biosynthetic model for us to understand the biosynthesis of thiopeptides.
语种: 英语
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WOS记录号: WOS:000275844200035
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/27799
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Wang J,Yu Y,Tang KX,et al. Identification and Analysis of the Biosynthetic Gene Cluster Encoding the Thiopeptide Antibiotic Cyclothiazomycin in Streptomyces hygroscopicus 10-22[J]. Appl. Environ. Microbiol.,2010,76(7):2335-2344.
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