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学科主题: 分析化学
题名: A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle AND LIFE SCIENCES
其他题名: 采用两性表面活性剂的部分填充胶束电动色谱作为简单的肽谱分析技术
作者: Huang J(黄靖) ; Kang JW(康经武)
通讯作者: 康经武
刊名: J. Chromatogr. B
发表日期: 2007-01-01T00:00:00Z
卷: 846, 期:1-2, 页:364-367
收录类别: SCI
部门归属: 中科院上海有机化学研究所; 中科院研究生院
英文摘要: A partial filling micellar electrokinetic capillary chromatography (PF-MEKC) method with a mixed micelle system composed of a zwitterionic surfactant named 3-(NN-dimethylhexadecylammonium)propanesulfonate (PAPS) and a nonionic surfactant polyethylene glycol dodecyl ether (Brij 35) for peptide mapping is described. The method was demonstrated by the separation of tryptic digestion of bovine serum albumin (BSA). The optimal mixed micelle solution was 50 mM NH4OH-HCOOH buffer (pH 2.0) containing 32 mM PAPS and 0.6% (m/v) Brij 35. It was found that the mixed micelle system permitted a highly selective separation of the cryptic digestion. The high separation selectivity was probably due to the ion-pairing interaction between the zwitterionic surfactant molecules and the peptides. (c) 2006 Elsevier B.V All rights reserved.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/27687
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Huang J,Kang JW. A simple peptide mapping method by partial filling micellar electrokinetic capillary chromatography with a zwitterionic-nonionic mixed micelle AND LIFE SCIENCES[J]. J. Chromatogr. B,2007,846(1-2):364-367.
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