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Screening of acetylcholinesterase inhibitors in natural extracts by CE with electrophoretically mediated microanalysis technique
其他题名电泳介导的毛细管电泳微分析技术用于天然产物提取物中乙酰胆碱酯酶抑制剂的筛选
Tang ZM(唐忠美); Wang ZY(王兆彦); Kang JW(康经武)
2007
发表期刊Electrophoresis
ISSN0173-0835
卷号28期号:3页码:360-365
摘要An electrophoretically mediated microanalysis (EMMA) method for screening acetylcholinesterase (AChE) inhibitors in natural extracts is described. In this method, solutions of AChE and the mixture of the substrate and the natural extract were successively injected into the capillary, and mixed electrophoretically by applying a voltage for a short time. Afterwards the voltage was reapplied to separate the product from the unreacted substrate and the natural extract. The measured peak area of the product at UV 230 nm represents the enzyme activity. Since the extract is mixed with the substrate, there is no need to separate the components before testing the inhibition. The inhibitory activity of the natural extract as a whole can be easily found if the peak area of the product is reduced. This makes the present method suitable for screening inhibitors in complex mixtures, such as natural extracts. Compared to the commonly used spectrometric method for screening of AChE inhibitors, the major advantage of the present method is the elimination of Ellman reagent, which is essential for the spectrometric method. This not only simplifies the experimental procedure but also minimizes false-positive results. Moreover, it is an obvious advantage of combining the separation power with the on-column enzyme assay for further investigating which compound(s) is/are responsible for the inhibition. The method was validated using a commercially available AChE inhibitor tacrine and a small chemical library containing four AChE inhibitors and 32 natural extracts. Inhibitors in natural extracts were identified with the present method.
学科领域分析化学
部门归属中科院上海有机化学研究所; 兰州大学化学化工学院; 中科院研究生院
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收录类别SCI
语种英语
WOS记录号WOS:000244425600012
引用统计
被引频次:41[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/27685
专题上海有机化学研究所
通讯作者Kang JW(康经武)
推荐引用方式
GB/T 7714
Tang ZM,Wang ZY,Kang JW. Screening of acetylcholinesterase inhibitors in natural extracts by CE with electrophoretically mediated microanalysis technique[J]. Electrophoresis,2007,28(3):360-365.
APA 唐忠美,王兆彦,&康经武.(2007).Screening of acetylcholinesterase inhibitors in natural extracts by CE with electrophoretically mediated microanalysis technique.Electrophoresis,28(3),360-365.
MLA 唐忠美,et al."Screening of acetylcholinesterase inhibitors in natural extracts by CE with electrophoretically mediated microanalysis technique".Electrophoresis 28.3(2007):360-365.
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