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学科主题: 分析化学
题名: Enantioseparation by CE with vancomycin as chiral selector: Improving the separation performance by dynamic coating of the capillary with poly(dimethylacrylamide)
其他题名: 万古霉素作为手性选择剂的毛细管电泳对映体分离:聚二甲基丙烯酰胺动态层对分离选择性的改善
作者: Wang ZY(王兆彦) ; Wang JF(王军锋) ; Hu ZD(胡之德) ; Kang JW(康经武)
通讯作者: 康经武
刊名: Electrophoresis
发表日期: 2007
卷: 28, 期:6, 页:938-943
收录类别: SCI
部门归属: 兰州大学化学化工学院; 中科院上海有机化学研究所; 陕西教育学院
英文摘要: An approach for improving the separation performance of the enantioseparation by CE with vancomycin as chiral selector is described. In the present method, a solution of poly(dimethylacrylamide) (PDMA) was used for dynamic coating of the capillary wall to minimize the adsorption of vancomycin onto the capillary wall, and to depress the EOF. Compared with the bare fused-silica capillaries and the capillaries coated with the polycationic polymer hexadimethrine bromide (HDB), the PDMA-coated capillary displayed the best separation performance. The resulting coating could withstand hundreds of runs without losing its function. Moreover, a partial filling technique was applied to avoid interference in detection caused by the presence of vancomycin in the buffer. The separation time was shortened when a short-end-injection technique was applied. Several parameters such as buffer pH, vancomycin concentration and plug length of the vancomycin solution for the separation were optimized. Under the optimal conditions, all tested enantiomers, including FMOC amino acids derivatives, ketoprofen and fenoprofen, were baseline-separated in less than 4.2 min.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000245717100008
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/27683
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Wang ZY,Wang JF,Hu ZD,et al. Enantioseparation by CE with vancomycin as chiral selector: Improving the separation performance by dynamic coating of the capillary with poly(dimethylacrylamide)[J]. Electrophoresis,2007,28(6):938-943.
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