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学科主题: 生命有机化学
题名: Crystallization and preliminary crystallographic study of rBmKαIT1, a recombinant α-insect toxin from the scorpion buthus martensii karsch
其他题名: 重组的马氏钳蝎α-昆虫毒素,BmkαIT1, 洁晶和初步的晶体学研究
作者: Huang T(黄旲) ; Huang QC(黄其辰) ; Chen HB(陈海宝) ; Tang YQ(唐有祺) ; Masami Kusunoki
通讯作者: 陈海宝
刊名: Acta Crystallogr. Sect. D-Biol. Crystallogr.
发表日期: 2003-01-01
卷: 59, 页:1635-1636
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家重点实验室; 北京大学化学院化学生物系; 日本大阪大学蛋白质研究所结构生物学研究中心
英文摘要: αInsect scorpion toxins are a distinct group of scorpion neurotoxins fro which no crystal structures are yet available. A novel αinsect toxin named BmKαIt1 from the scorpion Buthus martensii karsch (BmK) has been expressed as an inclusion body in Escherichia coli and purified by chromatography after renaturation. Recombinant BmKαIT1 (rBmKaIT1) was cystallized using the vapour-diffusion technique in hanging drops at 296 K. The crystals, which were grown in sodium phosphate, belonged to the orthorhombic space group P2^12^12^1. with unit-cell parameters α=30.24(1), b=36.51(3), c=57.08(2) A. Diffraction data were collected to 2.1 A resolution using synchrotron radiation. There appears to be one rBmKαIT1 molecule in the asymmetric unit.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/25753
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Huang T,Huang QC,Chen HB,et al. Crystallization and preliminary crystallographic study of rBmKαIT1, a recombinant α-insect toxin from the scorpion buthus martensii karsch[J]. Acta Crystallogr. Sect. D-Biol. Crystallogr.,2003,59:1635-1636.
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