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学科主题: 生命有机化学
题名: Two-cistron system overexpression of chloroplastglyceraldehyde-3-phosphate dehydrogenase subunit B and B-derivatives from spinach in escherichia coli
其他题名: 在大肠杆菌中双顺反子系统过表达菠菜叶绿体甘油醛-3-磷酸脱氧酶B亚基及其衍生物
作者: Tang GL(唐功利) ; Wang YF(王燕芳) ; Bao JS(鲍建绍) ; Chen HB(陈海宝)
通讯作者: 陈海宝
刊名: Protein Expr. Purif.
发表日期: 2001-01-01
卷: 22, 期:1, 页:31-37
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家重点实验室
英文摘要: A gene coding for the subnit B (GapB) of chloroplast glyceraldehyde-3-phosphate dehydrogenase from spinach and its two derivatives (GapBc) lacking the GapB-specific C-terminal extension have been cloned by RT-PCR. These three genes have been overexpressed with full activity in Escherichia coli when a two-cistron expression system controlled by an inducible promoter P trc is used. With a suitable base composition of the first cistron, the expression level of GapB and the derivatives GapBc are expressed up to 15-20% of the total cell protein and araround 20 mg of recombinant GapBcs with full activity are prigied from 1 00000liter of cultured bacteria. The specific activeity of the two derivatives GapBc(40-60 u/mg) is similar to that of GapA (50-70 u/mg) and lower than that of reported GapBc derivative (E. Baalmann, R. Scheibe, R. Cerff, and W. Martin, 1996, Plant Mol. Biol. 32, 505-513
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/25747
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Tang GL,Wang YF,Bao JS,et al. Two-cistron system overexpression of chloroplastglyceraldehyde-3-phosphate dehydrogenase subunit B and B-derivatives from spinach in escherichia coli[J]. Protein Expr. Purif.,2001,22(1):31-37.
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