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学科主题: 分析化学
题名: Mathematical model for DNA separation by capillary electrophoresis in entangled polymer solutions
其他题名: 使用毛细管无胶筛分电泳分离DNA的机理和数学模型
作者: Liu CY(刘春叶) ; Xu X(许旭) ; Wang Q(王前) ; Chen JR(陈杰瑢)
通讯作者: 许旭
刊名: J. Chromatogr. A
发表日期: 2007
卷: 1142, 期:2, 页:222-230
收录类别: SCI
部门归属: 西安交通大学生命科学与技术学院; 中国科学院上海有机化学研究所
英文摘要: A mathematical model of DNA separation by capillary electrophoresis in entangled polymer solution is presented. The mechanism is modeled as a DNA molecule moving through transient pores formed in polymer solutions and colliding with blobs of polymer molecules encountered during migration. By taking account of the average retardation time (t(c)) of DNA-blob collision and calculating the total collision number (N-c), a quantitative mathematical equation was reported, leading to predictions for the DNA mobility as a function of the experimental conditions like the size of DNA, the polymer concentration and the electric field strength. For DNA fragments in frequent size range, the initial experimental data agree well with the model. The DNA shape function (f(E)) was suggested and then discussed by the experimental data. The relationship between fE and electric field strength E was empirically estimated. Then, the average retardation time t(c) was obtained as about (2 similar to 3) x 10(-6) s in linear polyacrylamide (LPA) and hydroxyethylcellulose (HEC) solution. (c) 2006 Elsevier B.V. All rights reserved.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000244542400013
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/24765
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Liu CY,Xu X,Wang Q,et al. Mathematical model for DNA separation by capillary electrophoresis in entangled polymer solutions[J]. J. Chromatogr. A,2007,1142(2):222-230.
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