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Mathematical model for DNA separation by capillary electrophoresis in entangled polymer solutions
其他题名使用毛细管无胶筛分电泳分离DNA的机理和数学模型
Liu CY(刘春叶); Xu X(许旭); Wang Q(王前); Chen JR(陈杰瑢)
2007
发表期刊J. Chromatogr. A
ISSN0021-9673
卷号1142期号:2页码:222-230
摘要A mathematical model of DNA separation by capillary electrophoresis in entangled polymer solution is presented. The mechanism is modeled as a DNA molecule moving through transient pores formed in polymer solutions and colliding with blobs of polymer molecules encountered during migration. By taking account of the average retardation time (t(c)) of DNA-blob collision and calculating the total collision number (N-c), a quantitative mathematical equation was reported, leading to predictions for the DNA mobility as a function of the experimental conditions like the size of DNA, the polymer concentration and the electric field strength. For DNA fragments in frequent size range, the initial experimental data agree well with the model. The DNA shape function (f(E)) was suggested and then discussed by the experimental data. The relationship between fE and electric field strength E was empirically estimated. Then, the average retardation time t(c) was obtained as about (2 similar to 3) x 10(-6) s in linear polyacrylamide (LPA) and hydroxyethylcellulose (HEC) solution. (c) 2006 Elsevier B.V. All rights reserved.
学科领域分析化学
部门归属西安交通大学生命科学与技术学院; 中国科学院上海有机化学研究所
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收录类别SCI
语种英语
WOS记录号WOS:000244542400013
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被引频次:11[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/24765
专题上海有机化学研究所
通讯作者Xu X(许旭)
推荐引用方式
GB/T 7714
Liu CY,Xu X,Wang Q,et al. Mathematical model for DNA separation by capillary electrophoresis in entangled polymer solutions[J]. J. Chromatogr. A,2007,1142(2):222-230.
APA 刘春叶,许旭,王前,&陈杰瑢.(2007).Mathematical model for DNA separation by capillary electrophoresis in entangled polymer solutions.J. Chromatogr. A,1142(2),222-230.
MLA 刘春叶,et al."Mathematical model for DNA separation by capillary electrophoresis in entangled polymer solutions".J. Chromatogr. A 1142.2(2007):222-230.
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