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学科主题: 分析化学
题名: Simultaneous determination of baicalin, berberine and rhein in the traditional Chinese patent medicine San-Huang-Pian tablet by HPLC
其他题名: 高效液相色谱法同时测定三黄片中黄芩苷、小檗碱和大黄酸的含量
作者: Li Y(李奕) ; Xu X(许旭) ; Gao JP(高建平)
通讯作者: 许旭
刊名: J. Chin. Pharmaceut. Sci.
发表日期: 2005-01-01
卷: 14, 期:2, 页:110-114
部门归属: 中国科学院上海有机化学研究所; 金属化学国家重点实验室; 分析测试中心; 中国科学院研究生院 2. 上海中医药大学中药学院
英文摘要: Aim To establish a reversed phase liquid chromatographic method for simultaneous determination of three main medicinal constituents of baicalin, berberine and rhein in Sanhuang Tablets. Methods The separation was performed on a Kromasil C18 column with TEA-adjusted 0.02 mol?L-1 H3PO4 (pH 6.78)-acetonitrile-methanol (40:9:7) as mobile phase at a flow-rate of 1.0 ml?min-1, with detection at 254 nm. Considering interaction between acidic and alkaline compounds, three standard markers were added respectively and the volume of sample solution was expanded one time in recovery experiments. Results Three regression equations revealed excellent linear relationship between the peak areas and concentrations and the correlation coefficients all surpassed 0.999 8. The average recovery of baicalin is 96.1% (RSD = 2.1%), berberine 98.5% (RSD = 2.4%), rhein 101.5% (RSD = 1.3%). Conclusion The method developed can be used to control the quality of Sanhuang Tablets more comprehensively.
语种: 英语
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/24747
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
Li Y,Xu X,Gao JP. Simultaneous determination of baicalin, berberine and rhein in the traditional Chinese patent medicine San-Huang-Pian tablet by HPLC[J]. J. Chin. Pharmaceut. Sci.,2005,14(2):110-114.
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