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学科主题: 分析化学
题名: 毛细管电泳紫外检测条件下单碱基分离DNA片段
其他题名: DNA separation as single-base-pair resolution by capillary electrophoresis with ultraviolet detector
作者: 王前 ; 许旭
通讯作者: 许旭
刊名: 化学学报
发表日期: 2004-01-01
卷: 62, 期:1, 页:66-70
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所分析测试中心
摘要: 合成了一种水溶性聚合物, 聚N,N,二甲基丙烯酰胺(PDMA)和聚乙烯吡硌烷酮体系(PVP),优选该体系溶液含量为2.0% PDMA+2.0% PVP, 在紫外检测的条件下, 对标准DNA酶切片段pBR322/HaeIII样品进行分离, 其中的123/124进行分离, 分离度(Rs)为0.72.加入甲酰胺( ≥0.10)对DNA样品进行去活处理后,该体系亦能对单链DNA片段中的123/124进行分离, 分离度(Rs)为10.75。实验还显示进样时存在浓缩效应。 本体系粘度较小, 筛分能力强, 具备芯片毛细管DNA分离介质的潜力
英文摘要: The water-soluble mixtures of polymers, poly( N, N-dimethylacrylamide) (PDMA)/polyvinylpyrrolidone (PVP), were synthesized and used in capillary electrophoresis (CE) for DNA separation. With the optimal concentration of PDMA/PVP polymer solution, the doublet of 123/124 bp in pBR322/HaeIII Markers could be separated with UV detector and the resolution value was 0.72. When the denaturant formamide ( phi greater than or equal to 0. 10) was added in the polymer solution, DNA fragments of 123/124 could be also separated well with the resolution as 0.75, which quite met the requirement of DNA sequencing ( R-s > 0. 59). In addition, the lower viscosity of the polymer solution made the practice easier. The sample stacking effect was validated in the experiments. The medium showed single-base-pair DNA separation by capillary electrophoresis even with ultraviolet detector and uncoated capillary column.
语种: 中文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/24737
Appears in Collections:上海有机化学研究所_期刊论文

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Recommended Citation:
王前,许旭. 毛细管电泳紫外检测条件下单碱基分离DNA片段[J]. 化学学报,2004,62(1):66-70.
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