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Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein
Alternative Title具有抗HIV-1活性的新核糖体失活蛋白-栝楼蛋白
Zheng YT(郑永唐); Bi KL(贲昆龙); Jin SW(金善炜)
2000
Source PublicationActa Pharmacol. Sin.
ISSN1671-4083
Volume21Issue:2Pages:179-0182
AbstractTo determine whether trichobitacin, a novel ribosome-inactivating protein purified from the root tubers of trichosanthes kirlowii, possesses the anti-HIV activity. Methods: the inhibition of syncytial cell formaion induced by human immunodeficiency virus type 1 (HIV-1) was determined under microscope, reduction of HIV-1P24 antigen expression level was measured by ELISA, and decrease in numbers of HIV-1 antigen positive cells in acutely and chronically infected cultures were detected by indirect immunofluorescence assay. Results: Trichobitacin was found to greatly suppres syncytial cell formation induced by HIV-1 and to markedly reduce both expression HIV-1 p24 antigen and the number of HIV-1antigen positive cells in acutely but not chronically HIV-1 infected culture. The median inhibitory concentration (IC^50) in inhibition of syncytial cell formation and HIV antigen positive cells were 5 μg.L-1(95 % confidence limits: 1,3-20 μg.L-1) and 0.09 mg.L-~1(95% confidence limits: 0.011-0.755 mg.L~1), respectively. Conclusion: Trichobitacin is a novel ribosome-inactivating protein with anti-HIV-1activity.
Subject Area生命有机化学
Department中国科学院昆明动物研究所;中国科学院上海有机化学研究所
Indexed BySCI
Language英语
WOS IDWOS:000085208200014
Citation statistics
Document Type期刊论文
Identifierhttp://ir.sioc.ac.cn/handle/331003/24711
Collection上海有机化学研究所
Corresponding AuthorBi KL(贲昆龙)
Recommended Citation
GB/T 7714
Zheng YT,Bi KL,Jin SW. Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein[J]. Acta Pharmacol. Sin.,2000,21(2):179-0182.
APA 郑永唐,贲昆龙,&金善炜.(2000).Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein.Acta Pharmacol. Sin.,21(2),179-0182.
MLA 郑永唐,et al."Anti-HIV-1 activity of trichobitacin, a novel ribosome-inactivating protein".Acta Pharmacol. Sin. 21.2(2000):179-0182.
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