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The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase
其他题名酶反应诱导的甲醇脱氢酶中辅基PPQ(吡咯喹啉醌)的构象变化
Li J(李洁); Gan JH(甘建华); 斯科特, 马修斯; Xia ZX(夏宗芗)
2011
发表期刊Biochem. Biophys. Res. Commun.
ISSN0006-291X
卷号406期号:4页码:621-626
摘要Methanol dehydrogenase is a heterotetrameric enzyme containing the prosthetic group pyrroloquinoline quinone (PQQ), which catalyzes the oxidation of methanol to formaldehyde. The crystal structure of methanol dehydrogenase from Methylophilus W3A1, previously determined at high resolution, exhibits a non-planar configuration of the PQQ ring system and lends support for a hydride transfer mechanism of the enzymatic reaction catalyzed by the enzyme. To investigate why PQQ is in the C5-reduced form and to better understand the catalytic mechanism of the enzyme, three structures of this enzyme in a new crystal form have been determined at higher resolution. Two of the three crystals were grown in the presence of 1 and 50 mM methanol, respectively, both structures of which show non-planar configurations of the PQQ ring system, confirming the previous conclusion: the other was crystallized in the presence of 50 mM ethanol, the structure of which displays a planar ring system for PQQ. Comparison of these structures reveals that the configuration change of PQQ is induced by the enzymatic reaction. The reaction takes place and the C5-reduced PQQ intermediate is produced when the enzyme co-crystallizes with methanol, but the enzymatic reaction does not take place and the PQQ ring retains a planar configuration of the oxidized orthoquinone form when ethanol instead of methanol is present in the crystallization solution. (C) 2011 Elsevier Inc. All rights reserved.
学科领域生命有机化学
部门归属中科院上海有机化学研究所; 美国华盛顿大学医学院
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收录类别SCI
语种英语
WOS记录号WOS:000289036600023
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被引频次:8[WOS]   [WOS记录]     [WOS相关记录]
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/23700
专题生命有机化学国家重点实验室
通讯作者Xia ZX(夏宗芗)
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GB/T 7714
Li J,Gan JH,斯科特, 马修斯,et al. The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase[J]. Biochem. Biophys. Res. Commun.,2011,406(4):621-626.
APA 李洁,甘建华,斯科特, 马修斯,&夏宗芗.(2011).The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase.Biochem. Biophys. Res. Commun.,406(4),621-626.
MLA 李洁,et al."The enzymatic reaction-induced configuration change of the prosthetic group PQQ of methanol dehydrogenase".Biochem. Biophys. Res. Commun. 406.4(2011):621-626.
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