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Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS
其他题名Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS
REN AIMING; Xia ZX(夏宗芗); YU WENGONG; Zhou JH(周佳海)
2010
发表期刊Acta Crystallogr. F-Struct. Biol. Cryst. Commun.
ISSN1744-3091
卷号66页码:1635-1639
摘要AgaB from Pseudoalteromonas sp. CY24 is a novel agarase that hydrolyzes agarose to generate products with inverted anomeric configuration and that has been proposed to have a larger catalytic cleft than other beta-agarases. Here, the expression, purification, crystallization and data collection of AgaB in both wild-type and selenomethionine-substituted forms is described. The crystals of wild-type AgaB diffracted to 1.97 A resolution and belonged to space group C222(1). The selenomethionine derivative crystallized in space group I222. The phasing problem was solved by the multiwavelength anomalous dispersion (MAD) method. These results will facilitate detailed structural and enzymatic analysis of AgaB.
学科领域生命有机化学
部门归属中国科学院上海有机化学研究所; 中国海洋大学
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收录类别SCI
语种英语
文献类型期刊论文
条目标识符http://ir.sioc.ac.cn/handle/331003/23698
专题生命有机化学国家重点实验室
通讯作者Zhou JH(周佳海)
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GB/T 7714
REN AIMING,Xia ZX,YU WENGONG,et al. Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS[J]. Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,2010,66:1635-1639.
APA REN AIMING,夏宗芗,YU WENGONG,&周佳海.(2010).Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS.Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,66,1635-1639.
MLA REN AIMING,et al."Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS".Acta Crystallogr. F-Struct. Biol. Cryst. Commun. 66(2010):1635-1639.
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