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学科主题: 生命有机化学
题名: Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS
其他题名: Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS
作者: REN AIMING ; Xia ZX(夏宗芗) ; YU WENGONG ; Zhou JH(周佳海)
通讯作者: 周佳海
刊名: Acta Crystallogr. F-Struct. Biol. Cryst. Commun.
发表日期: 2010-01-01
卷: 66, 页:1635-1639
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所; 中国海洋大学
英文摘要: AgaB from Pseudoalteromonas sp. CY24 is a novel agarase that hydrolyzes agarose to generate products with inverted anomeric configuration and that has been proposed to have a larger catalytic cleft than other beta-agarases. Here, the expression, purification, crystallization and data collection of AgaB in both wild-type and selenomethionine-substituted forms is described. The crystals of wild-type AgaB diffracted to 1.97 A resolution and belonged to space group C222(1). The selenomethionine derivative crystallized in space group I222. The phasing problem was solved by the multiwavelength anomalous dispersion (MAD) method. These results will facilitate detailed structural and enzymatic analysis of AgaB.
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23698
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
REN AIMING,Xia ZX,YU WENGONG,et al. Expression, crystallization and preliminary X-ray analysis of an anomeric inverting agarase from Pseudoalteromonas sp CY24 COMMUNICATIONS[J]. Acta Crystallogr. F-Struct. Biol. Cryst. Commun.,2010,66:1635-1639.
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