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学科主题: 生命有机化学
题名: Structure of the F58W mutant of cytochrome b(5): the mutation leads to multiple conformations and weakens stacking interactions
其他题名: 细胞色素b5的F58W 突变体:突变导致多重构象并使堆积作用减弱
作者: LI SHAN ; LU JUNXIA ; GAN JIANHUA ; WANG YUNHUA ; HUANG ZHONGXIAN ; Xia ZX(夏宗芗)
通讯作者: HUANG ZHONGXIAN ; 夏宗芗
刊名: Acta Crystallogr. Sect. D-Biol. Crystallogr.
发表日期: 2005
卷: 61, 页:180-189
收录类别: SCI
部门归属: 上海有机化学研究所; 复旦大学
英文摘要: Phe58 of cytochrome b(5) is involved in stacking interactions with heme and the axial ligand His63. To elucidate the contribution of the stacking interactions to protein stability, the crystal structures of the F58Y and F58W mutants were determined at high resolution. The structure of the F58Y mutant is basically the same as that of the wild-type protein. However, the mutation from Phe58 to Trp58 leads to difficulty in growing single crystals and results in a space-group change; the six molecules m the asymmetric unit form two groups that are related by a non-crystallographic twofold axis. The structure of F58W was determined using molecular replacement by making use of the non-crystallographic symmetry. The F58W mutation gives rise to multiple conformations of six side chains, a peptide linking two of the six residues and the extended propionic acid of the heme. The six molecules in the asymmetric unit of the F58W mutant structure are grouped into two types based on their conformations and one of the six molecules exhibits dual conformations. The stacking interactions are weakened owing to the increase/decrease of the angles between the indole ring of Trp58 and the His63/heme rings, which accounts for the lower stability of F58W compared with the wild-type protein.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000226466900008
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23692
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
LI SHAN,LU JUNXIA,GAN JIANHUA,et al. Structure of the F58W mutant of cytochrome b(5): the mutation leads to multiple conformations and weakens stacking interactions[J]. Acta Crystallogr. Sect. D-Biol. Crystallogr.,2005,61:180-189.
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