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学科主题: 生命有机化学
题名: The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease
其他题名: 三维结构和X-射线法序列表明马干铃蛋白是新的类S-型核糖核酸酶
作者: Gan JH(甘建华) ; Yu L(俞璐) ; Wu J(邬键) ; Xu H(徐洪) ; Joyti S. Choudhary ; Walter P. Blackstock ; Liu WY(刘望夷) ; Xia ZX(夏宗芗)
通讯作者: 夏宗芗
刊名: Structure
发表日期: 2004
卷: 12, 期:6, 页:1015-1025
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所; Glaxo Smith Kline; 中国科学院上海生命科学院生物化学与细胞生物学研究所
英文摘要: Trichomaglin is a protein isolated from root tuber of the plant Maganlin (Trichosanthes Lepiniate, Cucurbitaceae). The crystal structure of trichomaglin has been determined by multiple-isomorphous replacement and refined at 2.2 Angstrom resolution. The X-ray sequence was established, based on electron density combined with the experimentally determined N-terminal sequence, and the sequence information derived from mass spectroscopic analysis. X-ray sequence-based homolog search and the three-dimensional structure reveal that trichomaglin is a novel S-like RNase, which was confirmed by biological assay. Trichomaglin molecule contains an additional beta sheet in the HVb region, compared with the known plant RNase structures. Fourteen cystein residues form seven disulfide bridges, more than those in the other known structures of S- and S-like RNases. His43 and His105 are expected to be the catalytic acid and base, respectively. Four hydrosulfate ions are bound in the active site pocket, three of them mimicking the substrate binding sites.
语种: 英语
相关网址: 查看原文
WOS记录号: WOS:000222155100013
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23686
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Gan JH,Yu L,Wu J,et al. The three-dimensional structure and X-ray sequence reveal that trichomaglin is a novel S-like ribonuclease[J]. Structure,2004,12(6):1015-1025.
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