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学科主题: 生命有机化学
题名: Proline-40 is essential to maintaining cytochrome b^5's stability and its electron transfer with cytochrome c
其他题名: Pro40是维持细胞色素b5的稳定性及其与细胞色素c之间的电子传递所必需的
作者: Wang ZQ(王志强) ; Wu J(邬键) ; Wang YH(王韵华) ; Qian W(钱雯) ; Xie Y(谢毅) ; Xia ZX(夏宗芗) ; Huang ZX(黄仲贤)
通讯作者: 夏宗芗
刊名: Chin. J. Chem.
发表日期: 2002-01-01
卷: 20, 期:11, 页:1212-1224
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机化学实验室; 复旦大学
英文摘要: In order to illustrate the roles played by Pro40 in the structure, properties and functions of cytochrome b5, three mutated genes, P40V, P40Y, P40G were constructed in this work. Only the P40V gene was succesfully expressed into holoprotein in E. coli JM83. According to the results of X-ray crystallographic analysis and various kinds of spectroscopy studies, it is evident that substituting valine for Pro40 does not result in significant alterations in the protein's overall structure; however, local conformational pertubations in the proximity of the heme do occur. The redox potential of the P40V mutant is 40 mV lower than that of the wild type protein. Its stability towards heat, urea, acid and ethanol were significantly decreased. The mutation leads to a decrease in the hydrophobicity of the heme pocket, which is probably the major factor contributing to the above changes. Binding constants and electron transfer rates between cytochrome b5 and cytochrome c were determined using UV-bisible spectroscopy and stopped-flow techniques for both the wild type and the utant. The results showed that the substitution of Pro40 by valine does not influence the binding constant of cytochrome b5 to cytochrome c; however, the electron tansfer rate between them devreased significantly. This indicates that proline-40 is essential to maintaining cytochrome b5's stability and its electron transfer with cytochrome c. These studies also provided a good example that property and functional changes of a protein do not necessarily require large overall structural alterations; in most cases, only perturbations on the local conformations are sufficient to induce significant changes in proteim's properties and functions.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23678
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Wang ZQ,Wu J,Wang YH,et al. Proline-40 is essential to maintaining cytochrome b^5's stability and its electron transfer with cytochrome c[J]. Chin. J. Chem.,2002,20(11):1212-1224.
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