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学科主题: 生命有机化学
题名: Structures of cytochrome b^5 mutated at the charged surface residues and their interactions with cytochrome c
其他题名: 细胞色素b5表面带电荷的残基突变后的结构及其与细胞色素C之间的相互作用
作者: Wu J(邬键) ; Wang YH(王韵华) ; Gan JH(甘建华) ; Wang WH(王文虎) ; Sun BY(孙炳耘) ; Huang ZX(黄仲贤) ; Xia ZX(夏宗芗)
通讯作者: 夏宗芗
刊名: Chin. J. Chem.
发表日期: 2002-01-01
卷: 20, 期:11, 页:1225-1234
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机化学实验室; 复旦大学
英文摘要: Glu44, Glu48, Glu56 and Asp60 are the negatively charged residues located at the molecular surface of cytochrome b5. Two mutants of cytochrome b5 were prepared, in which two or all of these four residues were mutated to alanines. The mutations give rise to slightly positive shifts of the redox potentials of cytochrome b5 and obvious decrease of the cytochrome b5-cytochrome c binding constants and electron transfer rates. The crystal structures of the two mutants were determined at 0.18 nm resolution, showing no alteration in overall structures and exhibiting slight changes in the local conformations around the mutation sites as compared with the wild-type protein. Based on the crystal structure of the quadruple-site mutant, a model for the binding of this mutant with cytochrome c is proposed, which involves the salt bridges from Glu37, Glu38 and heme propionate of cytochrome b5 to three lysines of cytochrome c and can well account for the properties and behaviors of this mutant.
语种: 英语
相关网址: 查看原文
内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23672
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Wu J,Wang YH,Gan JH,et al. Structures of cytochrome b^5 mutated at the charged surface residues and their interactions with cytochrome c[J]. Chin. J. Chem.,2002,20(11):1225-1234.
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