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学科主题: 生命有机化学
题名: Crystal structure of recombinant trypsin-solubilized fragment of cytochrome b^5 and the structural comparison with Val 61His Mutant
其他题名: 重组细胞色素 b5胰蛋白酶酶解片段的晶体结构及其与 Val 61His 突变体结构的比较
作者: Wu J(邬键) ; Gan JH(甘建华) ; Xia ZX(夏宗芗) ; Yuan-hua Wang ; Wen-Hu Wang ; Ling-LongXue ; Yi Xie ; Zhong-Xian Huang
通讯作者: 夏宗芗
刊名: Proteins: St
发表日期: 2000-01-01
卷: Funct., Genet. 2000, 40, 期:2, 页:249-257
收录类别: SCI
部门归属: 中国科学院上海有机化学研究所生命有机国家开放实验室; 上海复旦大学
英文摘要: The crystal structure of the recom-binant trypsin-solubilized fragment of the microso-tnal crochrome 6, from bovine liver has been deter-mined at 1.9 A resolution and compared with the reported crystal structure of the lipaso-solubilized fragment of the membrane protein cytoohrome b5 The two structures are similar to each other. However, some detailed structural differences are observed: the conformation of the segment Asnl6— Ser2O is quite different, some helices around the home and some segments between the helices are shifted slightly, the heme is rotated abont the nor-mal of the mean plane of home, one of the propionates of the home exhibits a different conformation: The average coordinatioa distances between the iron and the two nitrogen atoms of the imidazole ligands are the same in the two structures. Most or a the structural differences can be attributed to the different intermolecular interactions Which result from the crystal packicg. The wild-type protein structure is also compared with its Val6lHis mutarn, showing that the home binding and the main chain conformations are basically identical with each other except for the local area of the mutation site. However, when Val61 is mutated to bistidine. The large side chain of Hisdl is forced to point away from the home pocket toward the solvent region. Disturbing the micro-environment of the heme pocket and influencing the stability and the redox potential of the protein. Proteins
语种: 英语
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内容类型: 期刊论文
URI标识: http://ir.sioc.ac.cn/handle/331003/23666
Appears in Collections:生命有机化学国家重点实验室_期刊论文

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Recommended Citation:
Wu J,Gan JH,Xia ZX,et al. Crystal structure of recombinant trypsin-solubilized fragment of cytochrome b^5 and the structural comparison with Val 61His Mutant[J]. Proteins: St,2000,Funct., Genet. 2000, 40(2):249-257.
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